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AB249218

Anti-BTF antibody [EPR9981] - BSA and Azide free

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Rabbit Recombinant Monoclonal BTF antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1

2 이미지
Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)

This data was developed using ab155965, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized K562 cells using ab155965 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)
  • WB

Unknown

Western blot - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)

This data was developed using ab155965, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BTF antibody [EPR9981] (<a href='/ko/products/primary-antibodies/btf-antibody-epr9981-ab155965'>ab155965</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 106 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9981

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB237335

PE Anti-F4/80 antibody [EPR20548]

RabMAb|Recombinant

Flow Cytometry - PE Anti-F4/80 antibody [EPR20548] (AB237335)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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제품 세부 정보

ab249218 is the carrier-free version of ab155965.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The biological target BTF also known as BTF band or BTF 2 is a protein with an approximate mass of 42 kDa. It is highly expressed in various tissues including the brain and liver indicating its wide importance in cellular functions. BTF operates mechanically as a transcriptional regulator binding DNA sequences to control the transcription of specific genes. Additionally it interacts with other transcription factors assisting in the precise modulation of gene expression.
Biological function summary

BTF plays important roles in cellular proliferation and differentiation often as part of a larger transcriptional regulatory complex. Its activity helps to control gene expression during cell cycle progression and differentiation processes. The protein is essential for the accurate functioning of transcriptional machinery often being involved in the assembly of transcription pre-initiation complexes and modulating RNA polymerase activity.

Pathways

BTF acts centrally in the regulation of two significant pathways: the Wnt signaling pathway and the NF-kB signaling pathway. In both pathways BTF modulates the downstream transcription of responsive genes via its interaction with other pathway proteins like Beta-catenin in Wnt and RelA in NF-kB. These interactions help maintain proper cellular responses to extracellular signals ensuring balanced pathway activity and cellular responses.

BTF involvement has been linked to cancer and neurodegenerative diseases. Its dysregulation in transcriptional control can lead to the aberrant expression of genes related to oncogenesis or neuronal function. In cancer alterations in BTF activity may contribute to tumor progression and resistance to apoptosis through its connection with proteins like p53. In neurodegenerative diseases misregulation may affect neuronal growth and survival often interacting with proteins like tau which are linked to these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
See full target information BCLAF1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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