![Western blot - Anti-BTNL2 antibody [EPR12252] - BSA and Azide free (AB250420)](https://content.abcam.com/products/images/btnl2-antibody-epr12252-bsa-and-azide-free-ab250420--western-blot-img115658.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-BTNL2 antibody [EPR12252] - BSA and Azide free (AB250420)
This data was developed using ab181198, the same antibody clone in a different buffer formulation.
All lanes:
Western blot - Anti-BTNL2 antibody [EPR12252] (<a href='/ko/products/primary-antibodies/btnl2-antibody-epr12252-ab181198'>ab181198</a>) at 1/2000 dilution
Lane 1:
Human spleen tissue lysate at 20 µg
Lane 2:
Human tonsil tissue lysate at 20 µg
Lane 3:
Human small intestine tissue lysate at 20 µg
Lane 4:
K562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution
Predicted band size: 50 kDa
false
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-BTNL2 antibody [EPR12252]
보완 제품
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)](https://content.abcam.com/products/images/bob1-antibody-sp92-ab99891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121633.jpg)
Reactivity 정보
제품 세부 정보
ab250420 is the carrier-free version of ab181198.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
BTNL2 plays a significant role in modulating immune responses. It forms part of a complex with other immune checkpoint molecules providing inhibitory signals to T cells. This interaction helps maintain immune system balance and prevents excessive immune responses. The presence of BTNL2 in epithelial tissues suggests its involvement in mucosal immunity and interaction with the gut’s microbiome.
Pathways
BTNL2 directly participates in the immune checkpoint pathway. It negatively regulates the T-cell receptor signaling pathway in concert with proteins such as CTLA-4 and PD-1. These pathways are critical for maintaining immune homeostasis and avoiding autoimmunity by controlling T-cell activity.
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com