Anti-BTNL3 antibody [EPR30749-583]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human ileum tissue staining Wnt3 with ab325601 at a 1/2000 (0.246 μg/ml) dilution, ab272692 anti-MUC2 used at a 1/2000 (0.26 μg/ml) dilution, and ab324982 anti-BTNL3 used at a 1/500 (0.106 μg/ml) dilution.

Panel A : anti-Wnt3 (green; Opal™520), anti-MUC2 (magenta; Opal™690), anti-btnl3 (gray; Opal™570) on human ileum.

Panel B : anti-Wnt3 staining Paneth cells in human ileum.

Panel C : anti-MUC2 staining goblet cells in human ileum.

Panel D : anti-BTNL3 staining brush border in human ileum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325601, ab272692 and ab324982 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human ileum tissue staining BTNL3 with ab324982 at a 1/500 (0.106 μg/ml) dilution, ab272692 anti-MUC2 used at a 1/2000 (0.26 μg/ml) dilution and ab323315 anti-REG1B used at a 1/5000 (0.972 μg/ml) dilution.

Panel A : anti-BTNL3 (green; Opal™570), anti-MUC2 (magenta; Opal™690), anti-REG1B (gray; Opal™520) on human ileum.

Panel B : anti-BTNL3 staining brush border in human ileum.

Panel C : anti-MUC2 staining goblet cells in human ileum.

Panel D : anti-REG1B staining Paneth cells in human ileum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324982, ab272692 and ab323315 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human BTNL3 expression vector containing a his tag (B) HEK-293T cells transfected with a human BTNL8 expression vector containing a his tag (C) HEK-293T cells transfected with a human BTNL9 expression vector containing a his tag (D) HEK-293T cells transfected with empty vector containing a his tag tissue labeling BTNL3 with ab324982 at 1/500 (0.972 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a human BTNL3 expression vector containing a his tag, no staining on (B) HEK-293T cells transfected with a human BTNL8 expression vector containing a his tag, (C) HEK-293T cells transfected with a human BTNL9 expression vector containing a his tag and (D) HEK-293T cells transfected with empty vector containing a his tag.
The primary antibody was incubated with ab324982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling BTNL3 with ab324982 at 1/500 (0.972 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human liver.
The section was incubated with ab324982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling BTNL3 with ab324982 at 1/500 (0.972 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human cardiac muscle.
The section was incubated with ab324982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Immunohistochemical analysis of paraffin-embedded Human colorectal cancer and adjacent tissue labeling BTNL3 with ab324982 at 1/500 (0.972 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in adjacent colon tissue, with reduced expression in human colorectal cancer tissue.
The section was incubated with ab324982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL3 antibody [EPR30749-583] (AB324982)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling BTNL3 with ab324982 at 1/500 (0.972 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human small intestine.
The section was incubated with ab324982 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins