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AB237493

Anti-BUD31 antibody

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(1 출판물)

Rabbit Polyclonal BUD31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BUD31.

대체 명칭 보기

EDG2, BUD31, Protein BUD31 homolog, Protein EDG-2, Protein G10 homolog

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)

Paraffin-embedded human spleen tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (AB237493)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (AB237493)

MCF7 (human breast adenocarcinoma cell line) cells stained for BUD31 (green) using ab237493 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)

Paraffin-embedded human colon cancer tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BUD31.

P41223

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보완 제품

Primary Antibodies

AB181028

Anti-NADK2 antibody [EPR13170]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADK2 antibody [EPR13170] (AB181028)

  • 0
  • 0
Primary Antibodies

AB245571

Anti-USP39 antibody

Immunoprecipitation - Anti-USP39 antibody (AB245571)

  • 0
  • 0
Proteins & Peptides

AB156741

Recombinant Human BUD31 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human BUD31 protein (His tag N-Terminus) (AB156741)

  • 0
  • 0
Primary Antibodies

AB110252

Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (AB110252)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity greater than 95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

BUD31 also known as Bud Site Selection Protein 31 is a component of the spliceosome machinery and plays an important role in RNA splicing. It weighs about 28 kilodaltons. This protein is expressed in various tissues including the brain and the heart. BUD31 is necessary for proper spliceosome assembly and facilitates mRNA processing by aiding in the removal of introns during pre-mRNA splicing.
Biological function summary

BUD31 interacts with multiple proteins as part of the spliceosomal complex particularly during the catalytic steps of splicing. This interaction is essential for maintaining the fidelity of the splicing process preventing errors that could result in defective or dysfunctional mRNA transcripts. Researchers have observed BUD31 involvement in cellular processes such as cell division and proliferation indicating its importance in cell cycle regulation.

Pathways

Multiple researchers have linked BUD31 to the RNA splicing pathway which is an important step in gene expression. BUD31 also interacts with other spliceosome-associated proteins like SF3B1 highlighting its role in spliceosome assembly and function. Another important pathway is the regulation of the cell cycle where BUD31's influence on mRNA processing affects the expression of genes essential for cell cycle progression. This interaction may influence how cells respond to signals that promote growth or initiate division.

Scientists have linked mutations or dysregulation of BUD31 to cancer. Research suggests that BUD31 impacts the splicing of mRNAs that encode for oncogenes and tumor suppressor genes potentially affecting tumorigenesis. Additionally changes in BUD31 expression could relate to neurodegenerative conditions due to its significant expression in the brain and role in proper mRNA processing. Connections with proteins like BRCA1 in the context of cancer suggest BUD31's influence on cell cycle checkpoints and DNA repair mechanisms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Involved in the pre-mRNA splicing process (PubMed : 28076346, PubMed : 28502770). May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity (PubMed : 25091737).
See full target information BUD31
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:10111 PubMed39578459

2024

Histone lactylation mediated by Fam172a in POMC neurons regulates energy balance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Chen,Baocheng Wan,Hong Zhang,Lina Zhang,Rong Zhang,Lianxi Li,Yi Zhang,Cheng Hu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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