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AB243901

Anti-c-Maf antibody [BLR045F] - BSA free

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal c-Maf antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAF aa 100-200.

대체 명칭 보기

Transcription factor Maf, Proto-oncogene c-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, MAF

6 이미지
Immunocytochemistry - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)
  • ICC

Unknown

Immunocytochemistry - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)

Formalin-fixed, paraffin-embedded RPMI 8226 cells c-Mafa using ab243901 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)
  • IP

Unknown

Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)

c-Maf was immunoprecipitated from 1.0 mg RPMI 8226 whole cell lysate with ab243901 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243901 at 1/1000 dilution. Lane 1 : ab243901 IP in RPMI 8226 whole cell lysate.

Lane 2 : Control IgG in RPMI 8226 whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Predicted band size: 38 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)
  • WB

Unknown

Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)

Detection of human and mouse cMaf by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or cMaf using ab243901. Secondary : HRP-conjugated goat anti-rabbit IgG. Lower panel : Rabbit anti-Myc.

All lanes:

Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Predicted band size: 38 kDa

false

Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)
  • WB

Unknown

Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (AB243901)

Western blot analysis using ab243901 at 1/1000 dilution.

Lane 1 : HeLa cell whole cell lysate (50 µg).

Lane 2 : HEK-293T cell whole cell lysate (50 µg).

Lane 3 : A549 whole cell lysate (50 µg).

Lane 4 : RPMI 8226 whole cell lysate (50 µg).

Lane 5 : HepG2 whole cell lysate (50 µg).

Lane 6 : Jurkat whole cell lysate (50 µg).

Lane 7 : 786-O whole cell lysate (50 µg).

Lane 8 : MCF7 whole cell lysate (50 µg).

Lane 9 : K562 whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Predicted band size: 38 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR045F

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human MAF aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

O75444

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대체품

Primary Antibodies

AB203885

Anti-c-Maf antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody (AB203885)

  • 0
  • 0
Primary Antibodies

AB219213

Anti-c-Maf antibody [EPR16484] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] - BSA and Azide free (AB219213)

  • 0
  • 0
Primary Antibodies

AB199424

Anti-c-Maf antibody [EPR16484]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] (AB199424)

  • 0
  • 0
Primary Antibodies

AB225416

Alexa Fluor® 647 Anti-c-Maf antibody [EPR16484]

RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-c-Maf antibody [EPR16484] (AB225416)

  • 0
  • 0

보완 제품

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB75359

Anti-WHSC1/NSD2 antibody [29D1]

Trial Size

Western blot - Anti-WHSC1/NSD2 antibody [29D1] (AB75359)

  • 5
  • 4
Primary Antibodies

AB229766

Anti-MAFK antibody

Immunoprecipitation - Anti-MAFK antibody (AB229766)

  • 0
  • 0
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 6μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The c-Maf protein also known as Maf or MAF is a member of the large MAF family of basic leucine zipper (bZIP) transcription factors. This protein plays a significant role in the regulation of gene expression. It has a molecular weight of approximately 38 kDa. c-Maf is commonly expressed in various tissues such as the lens of the eye the kidneys and specific immune cells including T cells and macrophages. It is widely studied due to its involvement in transcriptional activation of certain target genes.
Biological function summary

C-Maf protein influences cellular differentiation and function particularly in immune cells. It forms part of a transcriptional complex that regulates genes critical for the production of interleukins and other cytokines. In the immune system c-Maf is essential for the differentiation of T-helper 2 cells and influences the production of cytokines such as IL-4. Additionally it is involved in the maturation of lens fibers in the eye and modulation of renal functions.

Pathways

C-Maf plays an important role in the immune and visual system. It is part of the signaling pathways that regulate Th2 cytokine genes including IL-4 within the adaptive immune response. This process involves interaction with other transcription factors such as STAT6 and GATA3. Moreover the lens development pathway also includes c-Maf where it works with proteins like CRYAA to maintain lens transparency and function.

C-Maf has been linked to multiple myeloma and juvenile idiopathic arthritis. c-Maf is often upregulated in multiple myeloma a cancer of plasma cells where it influences proliferation and survival of the malignant cells through its target genes like cyclin D2. Furthermore in juvenile idiopathic arthritis c-Maf contributes to the dysregulation of inflammatory cytokines indirectly involving proteins like IL-10 and IL-21. Understanding c-Maf’s function aids in exploring therapeutic targets for these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
See full target information MAF
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology research 33:1679-1693 PubMed40612868

2025

N6-Methyladenosine Promotes the Transcription of c-Src Kinase via IRF1 to Facilitate the Proliferation of Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxi Peng,Honggen Yuan,Zhanjie Jiang,Xiaoqing Ou,Qian Zhang,Kexin Yi,Yanbin Meng,Qun Xie

Genes & development 38:738-754 PubMed39168636

2024

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jiun C Chen,Govinal Badiger Bhaskara,Yue Lu,Kevin Lin,Sharon Y R Dent

Infectious agents and cancer 15:28 PubMed32391073

2020

Immune landscape in Burkitt lymphoma reveals M2-macrophage polarization and correlation between PD-L1 expression and non-canonical EBV latency program.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo Granai,Lucia Mundo,Ayse U Akarca,Maria Chiara Siciliano,Hasan Rizvi,Virginia Mancini,Noel Onyango,Joshua Nyagol,Nicholas Othieno Abinya,Ibrahim Maha,Sandra Margielewska,Wenbin Wi,Michele Bibas,Pier Paolo Piccaluga,Leticia Quintanilla-Martinez,Falko Fend,Stefano Lazzi,Lorenzo Leoncini,Teresa Marafioti
제품이 사용된 논문 모두 보기
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Product promise

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