Anti-c-Myc (phospho S62) antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (AB51156)

ICC/IF image of ab51156 stained HepG2 cells. The cells were 4% formlaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51156, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody (AB51156)

Immunohistochemical analysis of c-Myc (phospho S62) expression in paraffin-embedded human breast carcinoma tissue using 1/50 ab51156. left : untreated sample. Right : sample treated with phosphopeptide.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (AB51156)

Immunofluorescence analysis of HeLa cells, treated (left) and untreated (right) with Forskolin (40nM, 15mins), using c-Myc (phospho-Ser62) antibody.

• WB

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Western blot - Anti-c-Myc (phospho S62) antibody (AB51156)

All lanes:

Western blot - Anti-c-Myc (phospho S62) antibody (ab51156) at 1/500 dilution

Lane 1:

extracts from 293 cells treatedwith 40nM Forskolin for 30min, with phosphopeptide

Lane 2:

extracts from 293 cells treated with 40nM Forskolin for 30min, without phosphopeptide

Predicted band size: 48 kDa

Observed band size: 49 kDa

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