AB250554

Anti-C16orf70 antibody [EPR14035] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal C16orf70 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

C16orf6, C16orf70, PHAF1, Phagosome assembly factor 1

2 이미지

Supplier Data

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554)

This data was developed using ab181987, the same antibody clone in a different buffer formulation.

Immunoprecipitation of SH-SY5Y cell lysate, labeling C16orf70 usinf ab181987 at a 1/30 dilution (lane 1) or a negative control (lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/5000 dilution.

All lanes:

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] (<a href='/ko/products/primary-antibodies/c16orf70-antibody-epr14035-ab181987'>ab181987</a>)

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554)

This data was developed using ab181987, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C16orf70 antibody [EPR14035] (<a href='/ko/products/primary-antibodies/c16orf70-antibody-epr14035-ab181987'>ab181987</a>) at 1/1000 dilution

Lane 1:

Human fetal brain cell lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

JAR cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14035

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab250554 is the carrier-free version of ab181987.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

C16orf70 also known as Chromosome 16 Open Reading Frame 70 codes for a protein with a mass approximately of 17 kDa. This protein is mainly expressed in tissues such as the liver and lungs. C16orf70 may involve various functions in cellular processes but it lacks extensive characterization. Researchers aim to understand its role in different cellular contexts as it may hold significance in tissue-specific functions.

Biological function summary

The actions of C16orf70 suggest involvement in cellular signaling pathways. Studies hint at its participation in protein complexes although exact partners require further identification. Its presence in certain tissues implies a potential role linked to intercellular communication possibly influencing cell growth or immune responses. Further research continues to explore these biological interactions.

Pathways

C16orf70 might engage in critical roles within pathways like the Wnt signaling and cellular senescence. Its interactions with other proteins possibly including those from the Wnt pathway suggest involvement in regulating gene expression and maintaining cellular health. Understanding its precise function in these pathways will provide deeper insights into cell regulation and development.

Researchers link C16orf70 to conditions like cancer and chronic obstructive pulmonary disease (COPD). The protein's altered expression or function may interfere with cellular pathways highlighting associations with oncogenic or inflammatory processes. Studies investigate its connection with proteins involved in these disorders aiming to uncover potential therapeutic targets or biomarkers.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy.

See full target information PHAF1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C16orf70 antibody [EPR14035] - BSA and Azide free

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554)

Western blot - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise