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AB40765

Anti-CAD antibody [EP711Y]

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(2 제품이 사용된 논문 )

Rabbit Monoclonal CAD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD antibody [EP711Y] (AB40765)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD antibody [EP711Y] (AB40765)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma
using ab40765 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CAD antibody [EP711Y] (AB40765)
  • WB

Lab

Western blot - Anti-CAD antibody [EP711Y] (AB40765)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab40765 works better than ab40800 in western blot testing.

We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results when using ab955 in western blot.

Lanes 1 and 3:

Western blot - Anti-CAD antibody [EP710Y] (<a href='/ko/products/primary-antibodies/cad-antibody-ep710y-ab40800'>ab40800</a>) at 1/1000 dilution

Lanes 2 and 4:

Western blot - Anti-CAD antibody [EP711Y] (ab40765) at 1/1000 dilution

Lanes 1 - 2:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3 - 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 243 kDa

Observed band size: 250 kDa

false

Exposure time: 10s

Western blot - Anti-CAD antibody [EP711Y] (AB40765)
  • WB

Lab

Western blot - Anti-CAD antibody [EP711Y] (AB40765)

All lanes:

Western blot - Anti-CAD antibody [EP711Y] (ab40765) at 1/2000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 243 kDa

Observed band size: 245 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP711Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB99312

Anti-CAD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD antibody (AB99312)

  • 0
  • 0
Primary Antibodies

AB99313

Anti-CAD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD antibody (AB99313)

  • 4
  • 1
Primary Antibodies

AB40800

Anti-CAD antibody [EP710Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CAD antibody [EP710Y] (AB40800)

  • 0
  • 0
Primary Antibodies

AB97340

Anti-CAD antibody

Western blot - Anti-CAD antibody (AB97340)

  • 0
  • 0
Primary Antibodies

AB194534

Anti-CAD (phospho T456) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD (phospho T456) antibody (AB194534)

  • 0
  • 0
Primary Antibodies

AB203699

Alexa Fluor® 647 Anti-CAD antibody [EP710Y]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CAD antibody [EP710Y] (AB203699)

  • 0
  • 0

보완 제품

Primary Antibodies

AB231698

Anti-Lactate Dehydrogenase antibody [EP1563Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1563Y] - BSA and Azide free (AB231698)

  • 0
  • 0
Primary Antibodies

AB317032

Anti-Glutaminase antibody [EPR28716-72]

20ul selling size|Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody [EPR28716-72] (AB317032)

  • 0
  • 0
Primary Antibodies

AB178423

Anti-SDHB antibody [EPR13042(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (AB178423)

  • 5
  • 1
Primary Antibodies

AB129076

Anti-CPS1 antibody [EPR7493-3]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-3] (AB129076)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CAD protein also known as carbamoyl-phosphate synthase 2/ aspartate transcarbamylase/ dihydroorotase serves an important mechanical role in pyrimidine biosynthesis. CAD is a multi-functional enzyme with a molecular mass of approximately 243 kDa and is expressed in the cytoplasm of many cell types. This complex protein organization enables it to catalyze the first three steps of de novo pyrimidine synthesis involving glutamine-dependent carbamoyl phosphate synthesis aspartate transcarbamylation and dihydroorotase activity. Such vital tasks establish CAD as an important player in the nucleotide biosynthesis landscape.
Biological function summary

The CAD enzyme facilitates the production of pyrimidine nucleotides that are necessary for DNA and RNA synthesis. CAD operates as a single-polypeptide chain trifunctional enzyme and is part of a larger protein complex involved in nucleic acid metabolism. Its enzymatic activities align closely to support cellular proliferation and differentiation processes that demand efficient nucleotide production. This link between CAD function and nucleotide availability underlines its importance in ensuring smooth cell cycle progression and maintenance.

Pathways

CAD integrates into the broader nucleotide metabolism and pyrimidine biosynthesis pathways. The CAD protein directly interacts with other key enzymes such as UMP synthase which furthers the conversion of its product dihydroorotate to UMP. OMP decarboxylase also acts downstream reflecting CAD's role in ensuring continuity and efficiency in pyrimidine synthesis. These interactions demonstrate CAD's central involvement in maintaining the nucleotide pool required for DNA synthesis and repair.

CAD mutations and dysregulations link to neurological disorders and cancer. Neurological conditions such as CAD deficiency can result from impaired pyrimidine synthesis affecting brain development and function. Additionally enhanced CAD activity associates with certain cancers where its upregulated pyrimidine production supports rapid tumor cell proliferation. In these disease contexts CAD's interaction with proteins involved in cellular proliferation pathways such as c-Myc in cancers highlights its impact on health and disease states.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway : carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate.
See full target information CAD protein

대체 명칭 보기

Multifunctional protein CAD, Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase, CAD
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 20:e0330860 PubMed40901838

2025

The antiadipogenic effect of the pentacyclic triterpenoid isoarborinol is mediated by LKB1-AMPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yesenia Arcos-Reyes,Gildardo Rivera,Laurence A Marchat,Juan Salas-Benito,Gilberto Mandujano-Lázaro,Moisés Monzón-Gualito,Esther Ramírez-Moreno

Cancers 13: PubMed33670206

2021

Key Enzymes in Pyrimidine Synthesis, CAD and CPS1, Predict Prognosis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dirk Andreas Ridder,Mario Schindeldecker,Arndt Weinmann,Kristina Berndt,Lana Urbansky,Hagen Roland Witzel,Stefan Heinrich,Wilfried Roth,Beate Katharina Straub
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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