AB237754

Anti-Cadherin 7 antibody

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Rabbit Polyclonal Cadherin 7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDH7 aa 100-300.

대체 명칭 보기

CDH7L1, CDH7, Cadherin-7

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 7 antibody (AB237754)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 7 antibody (AB237754)

A549 (Human lung carcinoma cell line) cells stained for Cadherin 7 (green) using ab237754 at 1/133 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 7 antibody (AB237754)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 7 antibody (AB237754)

Paraffin-embedded human colon cancer tissue stained for Cadherin 7 using ab237754 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDH7 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q9ULB5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity greater than 95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cadherin 7 also known as CDH7 is a member of the classical cadherin family involved in calcium-dependent cell-cell adhesion. The mass of Cadherin 7 is approximately 88 kDa. It is mostly expressed in the nervous system where it plays a role in the formation and maintenance of cellular structures essential for neural function. The protein comprises extracellular cadherin repeats a transmembrane domain and a highly conserved cytoplasmic tail which allows it to facilitate homophilic interactions between cells.

Biological function summary

This protein mediates strong stable adhesion among cells in the neural tissue. It is integral to the assembly of adherens junctions where it participates in cell signaling and structural integrity. Cadherin 7 does not function alone; it often forms complexes with cytoplasmic proteins such as catenins which link the cadherin to the actin cytoskeleton providing mechanical support and facilitating intracellular signaling.

Pathways

Cadherin 7 plays a critical role in pathways like the cadherin-catenin complex and the Wnt signaling pathway. The cadherin-catenin complex pathway modulates cell polarity and gene expression by interacting with proteins like β-catenin. In the Wnt signaling pathway Cadherin 7 helps regulate cell fate proliferation and migration which are all vital for neural development and tissue homeostasis.

Cadherin 7 has implications in neurological conditions such as schizophrenia and autism spectrum disorders. Disruptions in its function may affect neural cell adhesion and signaling leading to these conditions. Additionally altered expression of Cadherin 7 in association with changes in other proteins like N-cadherin has been observed in cancer metastasis highlighting its importance in cell adhesion and signaling in both neural and cancer contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH7

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of gastroenterology 29:3770-3792 PubMed37426316

2023

Mechanism of ELL-associated factor 2 and vasohibin 1 regulating invasion, migration, and angiogenesis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Feng,Ming-Jun Sun,Bo-Yang Xu,Meng-Yuan Liu,Hui-Jing Zhang,Can Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cadherin 7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 7 antibody (AB237754)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 7 antibody (AB237754)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise