AB213652

Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Cadherin 9 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

대체 명칭 보기

Cadherin-9, CDH9

3 이미지

Flow Cytometry (Intracellular) - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

This data was developed using ab124803, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

This data was developed using ab124803, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cadherin 9 antibody [EPR5113] (<a href='/ko/products/primary-antibodies/cadherin-9-antibody-epr5113-ab124803'>ab124803</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 3:

Human testis lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa

Observed band size: 110 kDa

false

OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5113

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab213652 is the carrier-free version of ab124803.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cadherin-9 also known as CDH9 is a classical cadherin protein with a critical role in cell adhesion. This protein has a molecular mass of approximately 130 kDa. Cadherin-9 is part of the cadherin superfamily characterized by calcium-dependent cell-cell adhesion. It is expressed in neural tissues particularly within the brain where it facilitates neuron-neuron adhesion by forming adherens junctions. This adhesive function is essential for maintaining proper cellular architecture in tissues.

Biological function summary

Cadherin-9 contributes to the establishment and maintenance of cell-cell junctions. It is not part of a complex but associates strongly with other cadherin proteins maintaining tissue structure and signaling within cadherin-mediated junctions. The protein's adhesive properties are critical to neural development where it influences axonal guidance synapse formation and the stabilization of neuronal circuits. This role ensures accurate neural connectivity essential for proper brain functioning.

Pathways

The role of Cadherin-9 connects to key signaling pathways involving Wnt and calcium-dependent pathways. Within these pathways Cadherin-9 interacts with proteins like beta-catenin which links to the intracellular domain of cadherins at cell junctions and is involved in signal transduction. The interaction of Cadherin-9 within these pathways implicates it in the regulation of cellular activities like differentiation proliferation and cell polarity which are necessary for proper tissue development and homeostasis.

Cadherin-9 shows association with neurodevelopmental disorders such as autism and intellectual disabilities. In these conditions abnormal Cadherin-9 expression or function affects neuronal connectivity and synaptic stability. The protein also relates to the protein neuroligin which plays a role in synapse formation and is implicated in autism spectrum disorders. The Cadherin-9 and neuroligin interaction suggests pathways where disrupted cell adhesion and signaling impact neural circuitry formation and cognitive development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH9

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

Western blot - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (AB213652)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise