AB238158

Anti-Calcium Pump PMCA4 ATPase antibody

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Rabbit Polyclonal Calcium Pump PMCA4 ATPase antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP2B4 aa 250-350.

대체 명칭 보기

ATP2B2, MXRA1, ATP2B4, Plasma membrane calcium-transporting ATPase 4, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

Paraffin-embedded human colon cancer tissue stained for Calcium Pump PMCA4 ATPase with ab238158 at a 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

Paraffin-embedded human brain tissue stained for Calcium Pump PMCA4 ATPase with ab238158 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Calcium Pump PMCA4 ATPase (Green) using ab238158 at a 1/33 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ATP2B4 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P23634

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium Pump PMCA4 ATPase also known as ATP2B4 is a plasma membrane calcium ATPase responsible for the extrusion of Ca2+ ions from the cell. It plays an essential role in maintaining calcium homeostasis within eukaryotic cells. PMCA4 weighs approximately 134 kDa and is expressed in various tissues including the heart brain and kidney. The protein actively transports Ca2+ out of the cell using energy obtained from ATP hydrolysis.

Biological function summary

PMCA4 regulates intracellular calcium levels impacting processes such as muscle contraction neurotransmitter release and cell signaling. PMCA4 does not function as part of a larger protein complex but interacts with calmodulin which modulates its activity. Calmodulin binding activates PMCA4 increasing its capability to efflux Ca2+ and maintain cellular calcium balance.

Pathways

The calcium pump PMCA4 ATPase plays a critical role in the calcium signaling pathway and the regulation of cardiac rhythm. It helps control Ca2+ concentration within the cell affecting proteins like calcineurin that respond to calcium fluctuations. PMCA4 also interrelates with the sodium-calcium exchanger integrally involved in cardiac muscle contraction and relaxation.

PMCA4 has been linked to conditions such as heart failure and certain neurological disorders. Abnormal expression or function of PMCA4 can dysregulate calcium levels contributing to cardiac arrhythmias. Additionally altered PMCA4 activity can affect synaptic function connecting it indirectly to conditions like schizophrenia. Its interaction with proteins like alpha-synuclein implicated in neurological disorders further highlights PMCA4's relevance in disease states.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell (PubMed : 8530416). By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).

See full target information ATP2B4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cancer research and clinical oncology 150:294 PubMed38842683

2024

LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Fang Hou,Zhenghua Teng,Weiwei Xia,Jie Peng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calcium Pump PMCA4 ATPase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

Immunocytochemistry/ Immunofluorescence - Anti-Calcium Pump PMCA4 ATPase antibody (AB238158)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise