Anti-Calnexin antibody [CANX/1543] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [CANX/1543] - BSA and Azide free (AB237932)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for Calnexin using ab237932 at 2 μg/ml in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] - BSA and Azide free (AB237932)

This data was developed using the same antibody clone in a different buffer formulation (ab238078).

Lanes 1 - 4 : Merged signal (red and green). Green - ab238078 observed at 80 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab238078 was shown to react with Calnexin in wild-type HEK-293T cells in Western blot with loss of signal observed in CANX knockout cell line ab255368 (CANX knockout cell lysate ab263805). Wild-type HEK-293T and CANX knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab238078 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Calnexin antibody [CANX/1543] (<a href='/ko/products/primary-antibodies/calnexin-antibody-canx-1543-ab238078'>ab238078</a>) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

CANX knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human CANX (Calnexin) knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-canx-calnexin-knockout-hek-293t-cell-line-ab255368'>ab255368</a>)

Lane 3:

U-2 OS cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Predicted band size: 68 kDa

Observed band size: 80 kDa

false

• Protein Array

Unknown

Protein Array - Anti-Calnexin antibody [CANX/1543] - BSA and Azide free (AB237932)

This data was produced with ab238078, the same antibody in a different formulation with BSA and Azide.
ab238078 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• WB

Supplier Data

Western blot - Anti-Calnexin antibody [CANX/1543] - BSA and Azide free (AB237932)

All lanes:

Western blot - Anti-Calnexin antibody [CANX/1543] (<a href='/ko/products/primary-antibodies/calnexin-antibody-canx-1543-ab238078'>ab238078</a>) at 2 µg/mL

All lanes:

Human kidney lysate

Predicted band size: 68 kDa

false

• WB

Supplier Data

Western blot - Anti-Calnexin antibody [CANX/1543] - BSA and Azide free (AB237932)

All lanes:

Western blot - Anti-Calnexin antibody [CANX/1543] (<a href='/ko/products/primary-antibodies/calnexin-antibody-canx-1543-ab238078'>ab238078</a>) at 2 µg/mL

Lane 1:

PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 68 kDa

false