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AB324470

Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Calnexin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)

Interpolated concentration of native Calnexin was measured at different sample concentrations. Undiluted samples are as follows : HAP1 cell extract (3.1 µg/mL) and HEK293T cell extract (2.5 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)

Interpolated concentrations of native Calnexin in human control wild type HEK293T (human embryonic kidney epithelial cell) and CANX (Calnexin) knockout HEK293T cell based on 2.5 µg/mL extract loads. The concentrations of Calnexin were measured and interpolated from the Calnexin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The Calnexin concentration was determined to be 6233.8 pg/mL in wild type HEK293T extract and undetectable in CANX (Calnexin) knockout HEK293T extract (Human CANX (Calnexin) knockout HEK293T cell line ab255368).

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)

Sandwich ELISA with the capture antibody ab324470 dilution at 2 μg/mL and detector antibody ab324471 dilution at 0.5 μg/mL.

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)

Interpolated concentrations of native Calnexin in human control wild type HAP1 (human near-haploid cell) and CANX (Calnexin) knockout HAP1 cell based on 3.1 μg/mL extract loads. The concentrations of Calnexin were measured in duplicate and interpolated from the Calnexin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Calnexin concentration was determined to be 3829.6 pg/mL in wild type HAP1 extract and undetectable in CANX (Calnexin) knockout HAP1 extract.

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Calnexin antibody [EPR28028-138] - BSA and Azide free (Capture) (AB324470)

Interpolated concentration of native Calnexin was measured at different sample concentrations. Undiluted samples are as follows : human serum 100% and human plasma (EDTA) 100%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28028-138

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Proteins & Peptides

AB277017

Recombinant Mouse Calnexin protein (His tag)

SDS-PAGE - Recombinant Mouse Calnexin protein (His tag) (AB277017)

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Primary Antibodies

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Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

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Primary Antibodies

AB79559

Anti-CD81 antibody [M38]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [M38] (AB79559)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab324470 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Calnexin. The recommended pair for sandwich ELISA is:
Capture: ab324470, Human Calnexin Capture Antibody (unconjugated)
Detector: ab324471, Human Calnexin Detector Antibody (unconjugated)
The reference range value is 0.312-10 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
See full target information CANX

대체 명칭 보기

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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