AB28258

Anti-Calpain 1 antibody

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(6 리뷰들)

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(32 제품이 사용된 논문 )

Rabbit Polyclonal Calpain 1 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN1.

대체 명칭 보기

CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP

1 이미지

Western blot - Anti-Calpain 1 antibody (AB28258)

WB

Unknown

Western blot - Anti-Calpain 1 antibody (AB28258)

All lanes:

Western blot - Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution

All lanes:

Calpain 1 at 10 µg

Predicted band size: 82 kDa

Observed band size: 80 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

Immunogen

Synthetic Peptide within Human CAPN1. The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.).

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대체품

Primary Antibodies

AB108400

Anti-Calpain 1 antibody [EPR3319]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] (AB108400)

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1

Primary Antibodies

AB155677

Anti-Calpain 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB155677)

5
1

Primary Antibodies

AB39170

Anti-Calpain 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

0
0

Primary Antibodies

AB239930

Anti-Calpain 1 antibody [EPR3319] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] - BSA and Azide free (AB239930)

0
0

Primary Antibodies

AB28257

Anti-Calpain 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB28257)

5
1

보완 제품

Primary Antibodies

AB232559

Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (AB232559)

0
0

Primary Antibodies

AB28252

Anti-Calpastatin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calpastatin antibody (AB28252)

3
4

Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

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76

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody has been peptide-affinity purified.

보관 버퍼

Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.

Biological function summary

Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.

Pathways

Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.

Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed : 19617626, PubMed : 21531719, PubMed : 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed : 23707407). Cleaves and activates caspase-7 (CASP7) (PubMed : 19617626).

See full target information CAPN1

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제품이 사용된 논문 (32)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed40430436

2025

Epigallocatechin Gallate in Ameliorates Skin Aging by Reducing Mitochondrial ROS Production.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Ho Park,Eun Young Jeong,Ye Hyang Kim,So Yoon Cha,Ha Yeon Kim,Yeon Kyung Nam,Jin Seong Park,So Yeon Kim,Yoo Jin Lee,Jee Hee Yoon,Byeonghyeon So,Duyeol Kim,Minseon Kim,Youngjoo Byun,Yun Haeng Lee,Song Seok Shin,Joon Tae Park

Nature communications 14:8177 PubMed38071198

2023

Harnessing PROTAC technology to combat stress hormone receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mahshid Gazorpak,Karina M Hugentobler,Dominique Paul,Pierre-Luc Germain,Miriam Kretschmer,Iryna Ivanova,Selina Frei,Kei Mathis,Remo Rudolf,Sergio Mompart Barrenechea,Vincent Fischer,Xiaohan Xue,Aleksandra L Ptaszek,Julian Holzinger,Mattia Privitera,Andreas Hierlemann,Onno C Meijer,Robert Konrat,Erick M Carreira,Johannes Bohacek,Katharina Gapp

Experimental and therapeutic medicine 25:196 PubMed37090078

2023

Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Yan Wang,Jia-Xin Shi,Meng-Hua Chen,Xiang-Zhen Zhuge,Chun-Guang Dai,Lu Xie

International journal of molecular sciences 23: PubMed36499770

2022

Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models.

Applications

Unspecified application

Species

Unspecified reactive species

John A Miller,Domenica E Drouet,Leonid M Yermakov,Mahmoud S Elbasiouny,Fatima Z Bensabeur,Michael Bottomley,Keiichiro Susuki

iScience 24:103056 PubMed34755080

2021

Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Kang Tang,Yu-Hsien Lin,Joe-Air Jiang,Yun-Heng Lu,Chih-Hsuan Tsai,Yu-Chun Lin,Yun-Ru Chen,Carol-P Wu,Yueh-Lung Wu

International journal of molecular sciences 22: PubMed33919234

2021

Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Sukocheva,Dong Gui Hu,Robyn Meech,Anupam Bishayee

Biomedicines 9: PubMed33802221

2021

Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Wei Lu,Tzu-Yu Lin,Tai-Long Pan,Pei-Wen Wang,Kuan-Ming Chiu,Ming-Yi Lee,Su-Jane Wang

Doklady. Biochemistry and biophysics 495:338-341 PubMed33368047

2020

Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading.

Applications

Unspecified application

Species

Unspecified reactive species

Yu V Gritsyna,A D Ulanova,S S Popova,A G Bobylev,V K Zhalimov,T L Nemirovskaya,B S Shenkman,I M Vikhlyantsev

Biomedicine & pharmacotherapy = Biomedecine & phar 131:110788 PubMed33152946

2020

Prolyl oligopeptidase inhibition by KYP-2407 increases alpha-synuclein fibril degradation in neuron-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinar Rostami,Maria Jäntti,Hengjing Cui,Maiju K Rinne,Jyrki P Kukkonen,Anna Falk,Anna Erlandsson,Timo Myöhänen

Scientific reports 10:15185 PubMed32938992

2020

Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Popova,Anna Ulanova,Yulia Gritsyna,Nikolay Salmov,Vadim Rogachevsky,Gulnara Mikhailova,Alexander Bobylev,Liya Bobyleva,Yana Yutskevich,Oleg Morenkov,Nadezda Zakharova,Ivan Vikhlyantsev

제품이 사용된 논문 모두 보기

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Product promise

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