AB39170

Anti-Calpain 1 antibody

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(38 제품이 사용된 논문 )

Rabbit Polyclonal Calpain 1 antibody. Suitable for WB, ICC/IF, IHC (PFA fixed) and reacts with Human, Mouse, Rat samples. Cited in 38 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN1.

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Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC (PFA fixed), ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CAPN1. The exact immunogen used to generate this antibody is proprietary information.

P07384

Specificity

This antibody binds to reduced and non reduced Calpain 1. It reacts with Domain IV of Calpain 1, and detects both latent and aminoprocessed (activated) Calpain 1. It but does not cross react with the other calpain family members (Calpain 2, calpain 94, ncl 2, ncl 3, etc.).

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.

Biological function summary

Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.

Pathways

Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.

Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed : 19617626, PubMed : 21531719, PubMed : 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed : 23707407). Cleaves and activates caspase-7 (CASP7) (PubMed : 19617626).

See full target information CAPN1

대체 명칭 보기

CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP

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제품이 사용된 논문 (38)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bioengineering & translational medicine 10:e10737 PubMed40060768

2025

Ultrasound-mediated mechanical forces activate selective tumor cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ajay Tijore,Felix Margadant,Nehal Dwivedi,Leslie Morgan,Mingxi Yao,Anushya Hariharan,Claire Alexandra Zhen Chew,Simon Powell,Glenn Kunnath Bonney,Michael Sheetz

Cell communication and signaling : CCS 22:92 PubMed38303059

2024

T-type voltage-gated channels, Na/Ca-exchanger, and calpain-2 promote photoreceptor cell death in inherited retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yan,Lan Wang,Qian-Lu Yang,Qian-Xi Yang,Xinyi He,Yujie Dong,Zhulin Hu,Mathias W Seeliger,Kangwei Jiao,François Paquet-Durand

Frontiers in cellular neuroscience 17:1199656 PubMed37484825

2023

Prevention of noise-induced hearing loss by calpain inhibitor MDL-28170 is associated with upregulation of PI3K/Akt survival signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruosha Lai,Qiaojun Fang,Fan Wu,Song Pan,Khujista Haque,Su-Hua Sha

Cancer cell international 23:49 PubMed36932402

2023

Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maren Nicole Stillger,Chia-Yi Chen,Zon Weng Lai,Mujia Li,Agnes Schäfer,Axel Pagenstecher,Christopher Nimsky,Jörg Walter Bartsch,Oliver Schilling

International journal of molecular sciences 23: PubMed36233068

2022

Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model.

Applications

Unspecified application

Species

Unspecified reactive species

Elsayed Metwally,Hatim A Al-Abbadi,Mohamed A Hashem,Yasmina K Mahmoud,Eman A Ahmed,Ahmed I Maaty,Ibrahim E Helal,Mahmoud F Ahmed

Antioxidants (Basel, Switzerland) 11: PubMed35883722

2022

Mitochondrial Oxidative Stress Promotes Cardiac Remodeling in Myocardial Infarction through the Activation of Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco V Souza-Neto,Fabian Islas,Sara Jiménez-González,María Luaces,Bunty Ramchandani,Ana Romero-Miranda,Beatriz Delgado-Valero,Elena Roldan-Molina,Melchor Saiz-Pardo,Mª Ángeles Cerón-Nieto,Luis Ortega-Medina,Ernesto Martínez-Martínez,Victoria Cachofeiro

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5102-5114 PubMed35606145

2022

Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lee-Hsin Wang,Chien-Yu Lin,Yu-Mei Lin,Luc Buée,Nicolas Sergeant,David Blum,Yijuang Chern,Guey-Shin Wang

Bioengineering & translational medicine 6:e10233 PubMed34589605

2021

Enhanced tumor cell killing by ultrasound after microtubule depolymerization.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Singh,Ajay Tijore,Felix Margadant,Chloe Simpson,Deepak Chitkara,Boon Chuan Low,Michael Sheetz

Arteriosclerosis, thrombosis, and vascular biology 41:1694-1709 PubMed33761765

2021

Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Latha Muniappan,Michihiro Okuyama,Aida Javidan,Devi Thiagarajan,Weihua Jiang,Jessica J Moorleghen,Lihua Yang,Anju Balakrishnan,Deborah A Howatt,Haruhito A Uchida,Takaomi C Saido,Venkateswaran Subramanian

Kidney international 100:90-106 PubMed33675847

2021

Calpastatin prevents Angiotensin II-mediated podocyte injury through maintenance of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Imane Bensaada,Blaise Robin,Joëlle Perez,Yann Salemkour,Anna Chipont,Marine Camus,Mathilde Lemoine,Lea Guyonnet,Hélène Lazareth,Emmanuel Letavernier,Carole Hénique,Pierre-Louis Tharaux,Olivia Lenoir

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calpain 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody (AB39170)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (38)

Product promise