AB250819

Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

대체 명칭 보기

CASQ, CASQ1, Calsequestrin-1, Calmitine

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Calsequestrin 1 with ab185220 at 1/240 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/ko/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/10000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Lab

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentrartion : 5% NFDM/TBST. Exposure time : Lane 1 : 30 seconds; Lane 2 : 180 seconds. CASQ1 is the major isoform in adult fast-twitch skeletal muscles, while CASQ2 is the only isoform in cardiac muscle and a minor isoform in adult slow-twitch skeletal muscle (PMID : 33288873).

Lane 1:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/ko/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/10000 dilution

Lane 2:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/ko/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/2000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 63 kDa

false

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/ko/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/10000 dilution

Lane 1:

Rat muscle tissue lysate at 20 µg

Lane 2:

Mouse muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15226(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable for cell line testing in western blot.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab250819 is the carrier-free version of ab185220.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed : 28895244). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (PubMed : 28895244). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (PubMed : 27185316).

See full target information CASQ1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise