AB239859

Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CaMKI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, CAMK1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

Immunohistochemical analysis of Paraffin-embedded Human adrenal gland tissue labelling CaMKI with ab68234 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the zona glomerulosa of human adrenal glands. The section was incubated with ab68234 at +4°C overnight. Counter stained with Hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

ab68234 at 1/100-1/250 dilution staining CaMKI in human kidney tissue sections.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68234).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

Exposure time : Lane 1 & 3, 4, 5 : 5 seconds. Lane 2 & 6：20 seconds

Blocking and diluting buffer : 5% NFDM /TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68234).

All lanes:

Western blot - Anti-CaMKI antibody [EPR2217Y] (<a href='/ko/products/primary-antibodies/camki-antibody-epr2217y-ab68234'>ab68234</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Human brain tissue lysate at 40 µg

Lane 3:

Mouse brain tissue lysate at 40 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) at 40 µg

Lane 5:

Rat brain tissue lysate at 40 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2217Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

ab239859 is the carrier-free version of ab68234.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CaMKI also known as Calcium/Calmodulin-dependent protein kinase I is a serine/threonine-specific protein kinase with an approximate molecular mass of 42 to 58 kDa depending on the isoform. It plays an important mechanical role in converting calcium signals to cellular responses through phosphorylation of various substrates. This protein is ubiquitously expressed across different tissues including brain muscle and liver where it participates in diverse cellular processes.

Biological function summary

CaMKI functions as an essential mediator within the calcium/calmodulin-dependent protein kinase family. It modifies cellular activities by phosphorylating a range of downstream targets. Although CaMKI acts separately it associates with other family members like CaMKII sharing similar biochemical responses. In neurons it influences processes like synaptic plasticity and neurotransmitter release which are vital for learning and memory.

Pathways

It interacts within the calcium-signaling pathway and the MAPK/ERK pathway. Through calcium signaling CaMKI modulates gene transcription and apoptosis often working alongside other kinases like CaMKIV. In the MAPK/ERK pathway it plays a role in regulating cellular proliferation and differentiation ensuring proper cellular responses to external stimuli.

Alterations in CaMKI activity have links to neurological and cardiovascular conditions. Dysregulation of CaMKI especially in its interaction with proteins such as synapsin I is connected to cognitive impairments like those seen in neurodegenerative diseases. Moreover its involvement in heart tissue relates it to cardiac hypertrophy where inappropriate signaling can impact heart function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.

See full target information CAMK1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International archives of allergy and immunology 182:1245-1254 PubMed34428765

2021

Celastrol Exerts Cardioprotective Effect in Rheumatoid Arthritis by Inhibiting TLR2/HMGB1 Signaling Pathway-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Lu,Sha Gong,Xiaojun Wang,Nan Hu,Dan Pu,Jing Zhang,Yanhua Wang,Jing Luo,Qi An,Bomiao Ju,Lan He

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise