AB62215

Anti-CaMKI (phospho T177) antibody

Name: Anti-CaMKI (phospho T177) antibody
Brand: Abcam Limited
SKU: AB62215
Rating: 1 (1 reviews)

(1 리뷰)

(6 제품이 사용된 논문 )

Rabbit Polyclonal CaMKI phospho T177 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CAMK1 pT177 aa 100-200.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI (phospho T177) antibody (AB62215)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI (phospho T177) antibody (AB62215)

ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and ampli

Immunocytochemistry/ Immunofluorescence - Anti-CaMKI (phospho T177) antibody (AB62215)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CaMKI (phospho T177) antibody (AB62215)

Immunofluorescence analysis of HeLa cells, using ab62215 (1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CAMK1 pT177 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q14012

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab62215 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody againstnon phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CaMKI also known as Calcium/Calmodulin-dependent protein kinase I is a serine/threonine-specific protein kinase with an approximate molecular mass of 42 to 58 kDa depending on the isoform. It plays an important mechanical role in converting calcium signals to cellular responses through phosphorylation of various substrates. This protein is ubiquitously expressed across different tissues including brain muscle and liver where it participates in diverse cellular processes.

Biological function summary

CaMKI functions as an essential mediator within the calcium/calmodulin-dependent protein kinase family. It modifies cellular activities by phosphorylating a range of downstream targets. Although CaMKI acts separately it associates with other family members like CaMKII sharing similar biochemical responses. In neurons it influences processes like synaptic plasticity and neurotransmitter release which are vital for learning and memory.

Pathways

It interacts within the calcium-signaling pathway and the MAPK/ERK pathway. Through calcium signaling CaMKI modulates gene transcription and apoptosis often working alongside other kinases like CaMKIV. In the MAPK/ERK pathway it plays a role in regulating cellular proliferation and differentiation ensuring proper cellular responses to external stimuli.

Alterations in CaMKI activity have links to neurological and cardiovascular conditions. Dysregulation of CaMKI especially in its interaction with proteins such as synapsin I is connected to cognitive impairments like those seen in neurodegenerative diseases. Moreover its involvement in heart tissue relates it to cardiac hypertrophy where inappropriate signaling can impact heart function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.

See full target information CAMK1 pT177

대체 명칭 보기

Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, CAMK1

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biological & pharmaceutical bulletin 45:1354-1363 PubMed36047205

2022

Local Ca Signals within Caveolae Cause Nuclear Translocation of CaMK1α in Mouse Vascular Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiaki Suzuki,Tomo Kurata,Tsukasa Koide,Itsuki Okada,Nanami Nakajima,Yuji Imaizumi,Hisao Yamamura

Communications biology 5:124 PubMed35145192

2022

Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Mengjia Sun,Ran Cheng,Hu Tan,Chuan Liu,Renzheng Chen,Jihang Zhang,Yuanqi Yang,Xubin Gao,Lan Huang

Cell metabolism 30:508-524.e12 PubMed31204282

2019

Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Li,Chen-Song Zhang,Yue Zong,Jin-Wei Feng,Teng Ma,Meiqin Hu,Zhizhong Lin,Xiaotong Li,Changchuan Xie,Yaying Wu,Dong Jiang,Ying Li,Cixiong Zhang,Xiao Tian,Wen Wang,Yanyan Yang,Jie Chen,Jiwen Cui,Yu-Qing Wu,Xin Chen,Qing-Feng Liu,Jianfeng Wu,Shu-Yong Lin,Zhiyun Ye,Ying Liu,Hai-Long Piao,Li Yu,Zhuan Zhou,Xiao-Song Xie,D Grahame Hardie,Sheng-Cai Lin

The Journal of biological chemistry 292:14885-14901 PubMed28724635

2017

Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15()-hydroxyeicosatetraenoic acid-induced monocyte trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Sivareddy Kotla,Nikhlesh K Singh,Daniel Kirchhofer,Gadiparthi N Rao

Nature communications 8:33 PubMed28652571

2017

Discovery of long-range inhibitory signaling to ensure single axon formation.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuya Takano,Mengya Wu,Shinichi Nakamuta,Honda Naoki,Naruki Ishizawa,Takashi Namba,Takashi Watanabe,Chundi Xu,Tomonari Hamaguchi,Yoshimitsu Yura,Mutsuki Amano,Klaus M Hahn,Kozo Kaibuchi

Hippocampus 22:466-76 PubMed21298728

2011

A role for the CAMKK pathway in visual object recognition memory.

Applications

Unspecified application

Species

Unspecified reactive species

Chris J Tinsley,Katherine E Narduzzo,Malcolm W Brown,E Clea Warburton

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CaMKI (phospho T177) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI (phospho T177) antibody (AB62215)

Immunocytochemistry/ Immunofluorescence - Anti-CaMKI (phospho T177) antibody (AB62215)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise