Anti-CaMKII delta antibody [EPR13095]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1 : 100 dilution (1.82 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1 : 100 dilution (1.82 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Heat mediated antigen retrieval was performed citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CaMKII delta knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : A431 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)

Predicted band size: 56 kDa

false

• WB

Unknown

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution

Lane 1:

HeLa ( Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Mouse heart lysates at 20 µg

Lane 4:

Rat spleen lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

false

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

ab181052 was shown to react with CAMK2D in wild-type HAP1 cells in Western blot with loss of signal observed in a CAMK2D knockout cell line. Wild-type HAP1 and CAMK2D knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab181052 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 40 µg

Lane 2:

CAMK2D knock-out HAP1 lysate at 40 µg

false

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Lanes 1- 2 : Merged signal (red and green). Green - ab181052 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab181052 was shown to react with CaM-kinase II in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab267322 (knockout cell lysate ab257376) was used. Wild-type HEK-293T and CAMK2D knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab181052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human CAMK2D (CaMKII delta) knockout HEK-293T cell lysate (<a href='/ko/products/cell-lysates/human-camk2d-camkii-delta-knockout-hek-293t-cell-lysate-ab257376'>ab257376</a>) at 20 µg

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lanes 1 - 4:

Rat heart tissue lysate at 20 µg

Lanes 1 - 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 1 - 4:

SiHa (Human cervical squamous cell carcinoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 56 kDa,36 kDa

false

Exposure time: 60s

• WB

Supplier Data

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution

Lane 1:

SW480 whole cell lysate at 20 µg

Lane 2:

A431 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

• WB

CiteAb

Western blot - Anti-CaMKII delta antibody [EPR13095] (AB181052)

CaMKII delta western blot using anti-CaMKII delta antibody [EPR13095] ab181052. Publication image and figure legend from Qiu, H., Sun, Y., et al., 2024, Nat Commun, PubMed 39143095.

ab181052 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab181052 please see the product overview.

Genetic inhibition of SIRT1 in isogenic control iPSC-CMs activates ROS-CAMKII-RYR2 pathway and recapitulates pathogenic phenotypes of A388fs iPSC-CMs.a, b Western blot analysis of SIRT1, total CaMKII, ox-CaMKII, p-CaMKII, total RYR2, p-RYR2, and SUN1 expression in II-1-corr iPSC-CMs treated with scrambled siRNA (NC), SIRT1 siRNA (KD), or SIRT1 siRNA and SRT (KD + SRT), respectively. n = 4 independently biological repeats. c Cellular ROS levels in II−1-corr iPSC-CMs treated with scrambled siRNA, SIRT1 siRNA, or SIRT1 siRNA and SRT, respectively. n = 3 independently biological repeats. d Representative Ca2+ transient traces recorded from II−1-corr iPSC-CMs treated with scrambled siRNA, SIRT1 siRNA, or SIRT1 siRNA and SRT, respectively. e, f Bar graphs to compare the Ca2+ transient amplitude and diastolic [Ca2+]i among different groups in d. n = 19 (KD), 21 (NC, KD + SRT) cells. g Representative traces of cytosolic Ca2+ fluorescence in II−1-corr iPSC-CMs treated with scrambled siRNA, SIRT1 siRNA, or SIRT1 siRNA and SRT, respectively in NT solution and exposed to 0 Na+, 0 Ca2+ solution containing Tet and Caff. h, i Bar graphs to compare the RYR2-mediated SR Ca2+ leak and SR Ca2+ load among different groups in g. n = 24 (KD), 26 (KD + SRT), 30 (NC) cells. j Representative electrophysiological measurements of spontaneous action potentials in II-1-corr iPSC-CMs treated with scrambled siRNA, SIRT1 siRNA, or SIRT1 siRNA and SRT, respectively. k Bar graph to compare the occurrence of arrhythmias among different groups in j. l Representative graphs of staining of lamin A/C (green) and cardiac-specific marker TNNT2 (red) in II−1-corr iPSC-CMs treated with scrambled siRNA, SIRT1 siRNA, or SIRT1 siRNA and SRT, respectively. DAPI indicates nuclear staining (blue). Scale bar, 20 μm. m Bar graph to compare the occurrence of abnormal NE structure among different groups in l. n = 6 views from 3 independent experiments. Data are presented as mean ± SEM in b, c, e, f, h, i, m. p values were calculated by Dunnett’s multiple comparisons test in b, c, e, f, h, i, m. Source data are provided as a Source Data file.

false