AB59424

Anti-CAMKIV (phospho T196 + T200) antibody

Name: Anti-CAMKIV (phospho T196 + T200) antibody
Brand: Abcam Limited
SKU: AB59424
Rating: 2 (1 reviews)

(1 리뷰)

(11 제품이 사용된 논문 )

Rabbit Polyclonal CAMKIV phospho T196 + T200 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CAMK4 pT196 + T200 aa 150-250.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV (phospho T196 + T200) antibody (AB59424)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV (phospho T196 + T200) antibody (AB59424)

ab59424, at a 1/50 dilution, staining paraffin embedded human brain tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CAMK4 pT196 + T200 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q16566

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as CaMKIV (Calcium/Calmodulin-dependent Protein Kinase IV) plays a role in various cellular processes. Mechanically CaMKIV is a serine/threonine protein kinase with a molecular weight of approximately 52 kDa. It depends on calcium ions and calmodulin for activation. The protein is expressed in several tissues including the brain thymus and testis. It is known for its involvement in phosphorylating substrates which then activate transcription factors.

Biological function summary

CaMKIV takes part in the regulation of gene expression and is involved in neural signaling cell cycle control and immune responses. This kinase forms part of a larger signaling complex that facilitates specific cellular events. Its activity affects processes such as long-term potentiation and T-cell maturation indicating its significance in both the nervous and immune systems.

Pathways

CaMKIV integrates into several signaling pathways including the calcium signaling pathway and CREB (cAMP response element-binding protein) pathway. Through these pathways CaMKIV activates CREB via phosphorylation linking it to gene transcription processes essential for memory formation. CaMKIV acts alongside proteins like other CaM kinases such as CaMKI and CaMKII to ensure efficient signal transduction.

CaMKIV's function impacts conditions like neurodegenerative diseases and autoimmune disorders. Dysregulation of CaMKIV may relate to Alzheimer's disease where altered calcium homeostasis contributes to pathogenesis. The protein also connects to autoimmune diseases through its interaction with NFAT (nuclear factor of activated T-cells) which influences immune responses and might lead to disorders when dysregulated.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

See full target information CAMK4 pT196 + T200

대체 명칭 보기

CAMK, CAMK-GR, CAMKIV, CAMK4, Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 13:7529 PubMed36477027

2022

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Applications

Unspecified application

Species

Unspecified reactive species

S Mouron,M J Bueno,A Lluch,L Manso,I Calvo,J Cortes,J A Garcia-Saenz,M Gil-Gil,N Martinez-Janez,J V Apala,E Caleiras,Pilar Ximénez-Embún,J Muñoz,L Gonzalez-Cortijo,R Murillo,R Sánchez-Bayona,J M Cejalvo,G Gómez-López,C Fustero-Torre,S Sabroso-Lasa,N Malats,M Martinez,A Moreno,D Megias,M Malumbres,R Colomer,M Quintela-Fandino

Frontiers in immunology 13:890710 PubMed35734175

2022

CaMK4 Promotes Acute Lung Injury Through NLRP3 Inflammasome Activation in Type II Alveolar Epithelial Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Tengyue Zhang,Mengyuan Li,Siyuan Zhao,Mianjing Zhou,Huai Liao,Haiyan Wu,Xinyue Mo,Hongxing Wang,Chaohuan Guo,Hui Zhang,Niansheng Yang,Yuefang Huang

Cells 9: PubMed33291797

2020

Ca1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Moreno,Tamara Hermosilla,Paulina Hardy,Víctor Aballai,Patricio Rojas,Diego Varela

Acta pharmacologica Sinica 42:1055-1068 PubMed32868905

2020

DL0410 ameliorates cognitive disorder in SAMP8 mice by promoting mitochondrial dynamics and the NMDAR-CREB-BDNF pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Wen Lian,Wei Zhou,Bao-Yue Zhang,Hao Jia,Lv-Jie Xu,Ai-Lin Liu,Guan-Hua Du

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:14006-14023 PubMed32862457

2020

CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Sheng Zhan,Jia Chen,Jing Chen,Li-Gang Zhang,Yi Liu,He-Xi Du,Hui Wang,Mei-Juan Zheng,Zi-Qiang Yu,Xian-Guo Chen,Li Zhang,Chao-Zhao Liang

Bioscience reports 39: PubMed31769476

2019

Mechanism of AMPK-mediated apoptosis of rat gastric smooth muscle cells under high glucose condition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Zi Zhang,Mo-Han Zhang,Xue-Sen Fang,Xiang-Shu Cui,Zheng Jin

International journal of molecular sciences 20: PubMed31027360

2019

Inhibition of Calcium/Calmodulin-Dependent Protein Kinase Kinase β Is Detrimental in Hypoxia⁻Ischemia Neonatal Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Wei Min,Fan Bu,Li Qi,Yashasvee Munshi,Gab Seok Kim,Sean P Marrelli,Louise D McCullough,Jun Li

The Journal of biological chemistry 292:14885-14901 PubMed28724635

2017

Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15()-hydroxyeicosatetraenoic acid-induced monocyte trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Sivareddy Kotla,Nikhlesh K Singh,Daniel Kirchhofer,Gadiparthi N Rao

PloS one 8:e60863 PubMed23593332

2013

Stimulation of the sigma-1 receptor by DHEA enhances synaptic efficacy and neurogenesis in the hippocampal dentate gyrus of olfactory bulbectomized mice.

Applications

Species

Unspecified reactive species

Shigeki Moriguchi,Yasuharu Shinoda,Yui Yamamoto,Yuzuru Sasaki,Kosuke Miyajima,Hideaki Tagashira,Kohji Fukunaga

Hippocampus 22:466-76 PubMed21298728

2011

A role for the CAMKK pathway in visual object recognition memory.

Applications

Unspecified application

Species

Unspecified reactive species

Chris J Tinsley,Katherine E Narduzzo,Malcolm W Brown,E Clea Warburton

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAMKIV (phospho T196 + T200) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV (phospho T196 + T200) antibody (AB59424)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (11)

Product promise