AB234836

Anti-CAPZA1 antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal CAPZA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-1 aa 200 to C-terminus.

대체 명칭 보기

F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody (AB234836)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody (AB234836)

Paraffin-embedded human testis tissue stained for CAPZA1 using ab234836 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA1 antibody (AB234836)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA1 antibody (AB234836)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CAPZA1 (Green) using ab234836 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52907

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA1 also known as F-actin-capping protein subunit alpha-1 is a critical actin-binding protein with a molecular mass of approximately 33 kDa. It plays a role in the regulation of actin filament dynamics by capping the barbed ends of actin filaments effectively preventing further polymerization and depolymerization. CAPZA1 is expressed in a wide variety of tissues including skeletal muscle heart and brain indicating its importance in various cellular processes.

Biological function summary

The function of CAPZA1 influences the regulation of actin cytoskeleton organization. It is a part of the heterodimeric complex known as the F-actin-capping protein which includes beta subunit counterparts like CAPZB. This complex helps maintain the stability of actin filaments which are critical for cell motility shape and intracellular transport. CAPZA1's role in these cellular functions highlights its importance in processes ranging from muscle contraction to neuronal growth.

Pathways

CAPZA1 contributes significantly to the actin cytoskeleton pathway partnering with proteins such as gelsolin and profilin. Its activity affects cytoskeletal rearrangements which are integral to fundamental actions like cellular signaling adhesion and migration. The protein interacts in the Wnt signaling pathway where it may influence cell proliferation and differentiation processes through its modulation of actin filament dynamics.

CAPZA1 is linked to conditions like cardiac hypertrophy and certain cancers owing to its regulatory effect on actin filament dynamics. The disruptions in CAPZA1 expression can lead to aberrant cytoskeletal organization and cellular activity. Interactions with proteins such as vinculin are noted in these pathologies particularly as they relate to cell adhesion and migration in cancer metastasis. Understanding CAPZA1's role could provide insights into therapeutic targets for these diseases.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions (PubMed : 22891260). Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information F-actin-capping protein subunit alpha-1

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The American journal of pathology 193:1156-1169 PubMed37263345

2023

Development of Scaffold-Free Three-Dimensional Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Zhang,Konstantina Kyritsi,Abdulkadir Isidan,Yujin Park,Ping Li,Arthur A Cross-Najafi,Kevin Lopez,Lindsey Kennedy,Keisaku Sato,Shannon Glaser,Heather Francis,Gianfranco Alpini,Burcin Ekser

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAPZA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody (AB234836)

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA1 antibody (AB234836)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise