AB175378

Anti-CAPZA2 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal CAPZA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human F-actin-capping protein subunit alpha-2.

대체 명칭 보기

F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab175378. Blue DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-CAPZA2 antibody (AB175378)

All lanes:

Western blot - Anti-CAPZA2 antibody (ab175378) at 1/500 dilution

Lane 1:

HeLa lysate

Lane 2:

THP1 lysate

Lane 3:

22RV1 lysate

Lane 4:

Mouse brain lysate

Lane 5:

Mouse heart lysate

Predicted band size: 33 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human F-actin-capping protein subunit alpha-2.

P47755

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA2 also known as capping protein (actin filament) muscle Z-line alpha 2 is a protein that mechanically functions to regulate actin filament dynamics by binding to the barbed ends. This protein has an approximate molecular mass of 33 kDa. CAPZA2 is predominantly expressed in muscle tissues suggesting its significant role in muscle function. The protein forms part of the actin capping protein complex specifically interacting with the CAPZB subunit.

Biological function summary

CAPZA2 controls the growth of actin filaments an essential process for cell motility and stability. It is part of the capping protein complex which is necessary for the capping and stabilization of the fast-growing ends of actin filaments. This stabilization prevents further polymerization maintaining optimal filament length for cellular dynamics. CAPZA2 also plays a role in cytoskeletal organization important for muscle contraction and cell shape maintenance.

Pathways

The actin-regulating activity of CAPZA2 integrates into pathways involving actin cytoskeleton organization and dynamics. It is significantly involved in the actin-mediated processes interacting with proteins such as Arp2/3 complex which initiates actin branching. CAPZA2's role in cytoskeleton assembly also positions it within the signaling pathways related to cell growth and muscle function impacting interactions with proteins like tropomyosin which stabilizes actin filaments.

CAPZA2 shows a connection to muscular disorders including myopathy. Dysfunction in actin filament regulation can lead to issues in muscle fiber integrity and function contributing to muscle weakness or degenerative conditions. CAPZA2 is also linked to cardiac disorders due to its important role in muscle contractility interacting with proteins like myosin which is vital for heart muscle function. Understanding CAPZA2's roles can aid in developing therapeutic targets for muscle-related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

See full target information F-actin-capping protein subunit alpha-2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:1048 PubMed40659881

2025

Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Guo,Liming Liu,Xiao Mao,Manyu Xiao,Xiaobin He,Xing Pan,Yuewen Chen,Wanying Yi,Qibin Li,Xianglan Piao,Hua Wang,Yang Du,Yong Cheng

Cell death discovery 10:221 PubMed38719806

2024

Unique lipid composition maintained by extracellular blockade leads to prooncogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Kudo,Ryo Yanagiya,Masanori Hasegawa,Joaquim Carreras,Yoshimi Miki,Shunya Nakayama,Etsuko Nagashima,Yuji Miyatake,Kan Torii,Kiyoshi Ando,Naoya Nakamura,Akira Miyajima,Makoto Murakami,Ai Kotani

International journal of molecular sciences 20: PubMed31533312

2019

Freezing-Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Peris-Frau,Alicia Martín-Maestro,María Iniesta-Cuerda,Irene Sánchez-Ajofrín,Lourdes Mateos-Hernández,J Julián Garde,Margarita Villar,Ana Josefa Soler

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAPZA2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Western blot - Anti-CAPZA2 antibody (AB175378)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise