- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] - BSA and Azide free (AB250486)
This data was developed using ab181358, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Carbonic Anhydrase 3/CA3 with ab181358 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] - BSA and Azide free (AB250486)
This data was developed using ab181358, the same antibody clone in a different buffer formulation.
All lanes:
Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (<a href='/ko/products/primary-antibodies/carbonic-anhydrase-3-ca3-antibody-epr13425-ab181358'>ab181358</a>) at 1/1000 dilution
Lane 1:
Human skeletal muscle lysates at 10 µg
Lane 2:
Rat muscle lysates at 10 µg
Lane 3:
Mouse muscle lysates at 10 µg
Predicted band size: 30 kDa
false
관련 conjugated 항체와 다양한 조성의 항체 (1)
-
Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425]
Reactivity 정보
제품 세부 정보
ab250486 is the carrier-free version of ab181358.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com