Anti-Carbonic Anhydrase 9/CA9 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB15086)

Immunohistochemistry analysis of a formalin-fixed, paraffin-embedded tissue section of human breast cancer using ab15086 at 1 : 1000 dilution. The primary antibody bound to Carbonic Anydrase 9/CA9 antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated an expected cytoplasmic staining of Carbonic Anydrase 9/CA9 protein with an intense signal around the cellular membranes in tumor cores. The latter are more likely to be hypoxic in growing tumors which signifies that the observed Carbonic Anydrase 9/CA9 staining is specific.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB15086)

Immunohistochemistry analysis of human renal cell cancer tumor cryosections stained with ab15086 at a 1/500 dilution (left) or normal rabbit serum (right).

• WB

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Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB15086)

Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in rat renal cortex lysate.

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)

Predicted band size: 49 kDa

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• IHC

CiteAb

Immunohistochemistry - Anti-Carbonic Anhydrase 9/CA9 antibody (AB15086)

Immunohistochemistry using Anti-Carbonic Anhydrase 9/CA9 antibody, ab15086. Publication image from Zuffardi, O. et al., 2009, Mol Cancer, 19642973. Legend direct from paper.

Immunohistochemical analysis of antigens in dysgerminoma. (A) normal Fallopian tube; (B-H) dysgerminoma. (A) intense TRC8 immunoperoxidase expression in control tissue. (B) negative immunoperoxidase TRC8 reaction in dysgerminoma; C, CD117 strong membrane staining in dysgerminoma; D, diffuse and intense VHL expression; E, FGFR cytoplasmic expression; F, diffuse and intense CAIX expression; G, HIF2alpha nuclear and cytoplasmic expression in sporadic cells; H, moderate lipid deposition detected by Oil Red O. Original magnification A-H x100.

• WB

CiteAb

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB15086)

Carbonic Anhydrase 9/CA9 western blot using anti-Carbonic Anhydrase 9/CA9 antibody ab15086. Publication image and figure legend from Sousa, B., Ribeiro, A. S., et al., 2014, BMC Cancer, PubMed 25269858.

ab15086 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab15086 please see the product overview.

MFE (%) decreases upon siRNA-mediated silencing ofCDH3,HIF-1α, GLUT1andCAIXin SUM149 cells. siRNA-mediated silencing of CDH3, HIF-1α, GLUT1 and CAIX was confirmed by western blot analysis (A). A statistically significant decrease in MFE (%) was observed when we silenced CDH3, HIF-1α, GLUT1 and CAIX. The simultaneous silencing of CDH3 and HIF-1α also revealed a significant decrease of MFE (%) of the target cells, although not cumulative. Still, the silencing of the expression of all transcripts tested led to a non-cumulative inhibition of the MFE compared to the control cells (B). *p < 0.05.

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