Anti-Carboxymethyl Lysine antibody [CML26]
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(21 제품이 사용된 논문 )
Anti-Carboxymethyl Lysine antibody [CML26] (ab125145) is a mouse monoclonal antibody detecting Carboxymethyl Lysine in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA.
- Over 10 publications
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CML, N Epsilon (Carboxymethyl) Lysine
- Flow Cyt
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Flow Cytometry - Anti-Carboxymethyl Lysine antibody [CML26] (AB125145)
Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Reactivity 정보
제품 세부 정보
Anti-Carboxymethyl Lysine antibody [CML26] (ab125145) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA. Trusted by the scientific community
Anti-Carboxymethyl Lysine [CML26] (ab125145) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
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제품이 사용된 논문 (21)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Journal of cosmetic dermatology 24:e70521 PubMed41118451
2025
Applications
ICC/IF, IHC-Fr
Species
Human, Human
Scientific reports 15:35226 PubMed41068344
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of nanomedicine 20:11417-11434 PubMed40989892
2025
Applications
Unspecified application
Species
Unspecified reactive species
Arteriosclerosis, thrombosis, and vascular biology 45:424-442 PubMed39817328
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of biological sciences 20:880-896 PubMed38250151
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cells 12: PubMed38067186
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cells 12: PubMed37174618
2023
Applications
Unspecified application
Species
Unspecified reactive species
World journal of diabetes 14:222-233 PubMed37035231
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biomolecules 13: PubMed36979358
2023
Applications
Unspecified application
Species
Unspecified reactive species
World journal of diabetes 13:972-985 PubMed36437860
2022
Applications
Unspecified application
Species
Unspecified reactive species
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