AB151560

Anti-CARD6 antibody [EPR9718]

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CARD6 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

2 이미지

Flow Cytometry (Intracellular) - Anti-CARD6 antibody [EPR9718] (AB151560)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CARD6 antibody [EPR9718] (AB151560)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling CARD6 with ab151560 at 1/10 dilution (red), compared to a Rabbit IgG control antibody (green).

Unknown

Western blot - Anti-CARD6 antibody [EPR9718] (AB151560)

All lanes:

Western blot - Anti-CARD6 antibody [EPR9718] (ab151560) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

293T lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 116 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9718

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CARD6 also known as Caspase Recruitment Domain-Containing Protein 6 is a protein that weighs approximately 104 kDa. The protein is identified for its involvement in the immune response and connects with microtubules in the cell. It is expressed in various tissues including the heart brain and skeletal muscles. CARD6 contains a CARD (Caspase Activation and Recruitment Domain) enabling it to interact with other proteins containing similar domains.

Biological function summary

CARD6 influences immune system regulation playing a role in apoptosis and inflammation. It does not form a complex with other proteins but instead interacts directly with several proteins through its CARD domain. Notably CARD6 modulates the functions of NF-kB a transcription factor important for immune response regulation and cellular stress responses.

Pathways

CARD6 participates in the NF-kB signaling pathway and the interferon regulatory pathway. These pathways are essential for mediating immune responses and controlling inflammation within the body. CARD6 shows interaction with proteins like RIP2 and TRAF6 within these pathways which further affect the NF-kB signaling cascade.

CARD6 has been linked to Crohn's disease and certain cancers. Abnormalities in its function or expression can lead to dysregulated NF-kB activity contributing to these conditions. CARD6 interacts with proteins such as RIP2 in autoimmune disorders like Crohn's disease and its altered signaling pathway connections can play a role in cancer progression by affecting cell survival and proliferation mechanisms.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

May be involved in apoptosis.

See full target information CARD6

대체 명칭 보기

Caspase recruitment domain-containing protein 6, CARD6

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Exploration (Beijing, China) 5:e20230178 PubMed40873646

2025

Enhanced Blood-Brain Barrier Penetrability of BACE1 SiRNA-Loaded Prussian Blue Nanocomplexes for Alzheimer's Disease Synergy Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Ding,Yanyu Hu,Xiaotong Feng,Zekun Wang,Qile Song,Chunxue Dai,Bangjia Yang,Xiaoyan Fu,Dongdong Sun,Cundong Fan

Aging 11:12213-12235 PubMed31841440

2019

Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Lin Wang,Xiao Luo,Li Liu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CARD6 antibody [EPR9718]

Flow Cytometry (Intracellular) - Anti-CARD6 antibody [EPR9718] (AB151560)

Western blot - Anti-CARD6 antibody [EPR9718] (AB151560)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise