AB47003

Anti-Cardiac Troponin I antibody

Name: Anti-Cardiac Troponin I antibody
Brand: Abcam Limited
SKU: AB47003
Rating: 5 (17 reviews)

BOND RX™ Validated
각 태그의 뜻

(17 리뷰들)

(174 제품이 사용된 논문 )

Anti-Cardiac Troponin I antibody (ab47003) is a rabbit polyclonal antibody detecting Cardiac Troponin I in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Pig, Rat.

- Over 140 publications
- Trusted since 2007

6 이미지

Project

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/ko/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/ko/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB47003)

ICC/IF

AbReview29464****

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB47003)

ab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Lab

Immunohistochemistry - Anti-Cardiac Troponin I antibody (AB47003)

Immunohistochemical analysis of formalin fixed paraffin embedded human heart labelling cardiac Troponin I with ab47003 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab47003 anti-Cardiac Troponin I antibody was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB47003)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB47003)

IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

AbReview15169****

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Blocking Step : 20% Serum for 3 hours at 24°C

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution

All lanes:

Pig Heart whole cell lysate at 4 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 29 kDa

true

This image is courtesy of an Abreview submitted by Dr. Mario Torrado

Unknown

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/ko/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 3:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 24 kDa

Observed band size: 160 kDa,26 kDa,80 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

Flow Cyt, WB, ELISA, IHC-P, ICC/IF, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Anti-Cardiac Troponin I antibody (ab47003) is a rabbit polyclonal antibody and is validated for use in ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB in african green monkey, human, mouse, rat samples.

Anti-Cardiac Troponin I antibody (ab47003) has been cited over 147 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Cardiac Troponin I antibody (ab47003) has high sensitivity and specificity.

Anti-Cardiac Troponin I antibody (ab47003) has 17 independent reviews from customers.

Anti-Cardiac Troponin I antibody (ab47003) specifically detects Cardiac Troponin I (UniProt ID: P19429; Molecular weight: 24kDa) and is sold in a 100 µg selling size.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI3

대체 명칭 보기

TNNC1, TNNI3, Cardiac troponin I

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제품이 사용된 논문 (174)

Recent publications for all applications. Explore the 전체 목록 and refine your search

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Yuhua Wei,Xiaoxiao Geng,Qing You,Yu Zhang,Fangfang Cao,Gunaseelan Narayanan,Thanh Nguyen,Xiaoyuan Chen,Jianyi Zhang,Lei Ye

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Macrophage Notch1 drives septic cardiac dysfunction by impairing mitophagy and promoting NLRP3 activation.

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Yanjun Zheng,Jingrong Lin,Guoqing Wan,Xuefeng Gu,Jian Ma

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Prakaimuk Saraithong,Peyton Krajcarski,Yukako Kusaka,Moe Yamada,Junichi Matsumoto,Hailey Cunningham,Sama Salih,Darby Jones,Devika Baddhan,Christian Hausner,Justus Anumonwo,Anthony Rosenzweig,Mary M Navarro,Luis Villa Diaz,Joseph Criscione,Deok-Ho Kim,Todd J Herron

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Species

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제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin I antibody

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (AB47003)

Immunohistochemistry - Anti-Cardiac Troponin I antibody (AB47003)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB47003)

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (174)

Product promise