Anti-Cardiac Troponin T antibody [1F11]

• sELISA

Unknown

Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (AB10214)

Standard Curve for Cardiac Troponin T (Analyte : Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5μg/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5μg/ml.

• ICC

Lab

Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (AB10214)

Immunofluorescence staining of Cardiac Troponin T using ab10214 in ab277612, which were differentiated for 10 days post induction.

The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 μg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).