AB242361

Anti-CARS antibody [CL2302]

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Mouse Monoclonal CARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CARS1 aa 500 to C-terminus.

대체 명칭 보기

CARS, CARS1, Cysteinyl-tRNA synthetase, CysRS

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human cerebellum tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Negative control :

Paraffin-embedded human skeletal muscle tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human breast cancer tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human pancreas tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Paraffin-embedded human fallopian tube tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-CARS antibody [CL2302] (AB242361)

All lanes:

Western blot - Anti-CARS antibody [CL2302] (ab242361) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction

Lane 2:

HeLa membrane fraction

Lane 3:

HeLa nuclear fraction

Lane 4:

HeLa chromatin fraction

Lane 5:

HeLa cytoskeletal fraction

Predicted band size: 85 kDa

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Supplier Data

Western blot - Anti-CARS antibody [CL2302] (AB242361)

All lanes:

Western blot - Anti-CARS antibody [CL2302] (ab242361) at 1 µg/mL

All lanes:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 85 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2302

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CARS1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49589

Epitope

Binds to an epitope located within the peptide sequence RPNQALLENIALYLT as determined by overlapping synthetic peptides.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CARS protein also known as cysteinyl-tRNA synthetase is an enzyme responsible for attaching cysteine to its corresponding tRNA thereby playing a vital role in protein synthesis. The molecular mass of CARS is approximately 87 kDa and it is widely expressed in various human tissues including the liver and brain. As a part of the aminoacyl-tRNA synthetase (aaRS) family it ensures that the genetic code is accurately translated into functional proteins.

Biological function summary

Cysteinyl-tRNA synthetase acts as an important component in the translation machinery ensuring incorporation of cysteine into growing polypeptide chains during protein synthesis. It forms part of the multi-synthetase complex (MSC) which is important for cellular translation and efficiency. Beyond its primary synthetase activity CARS also participates in additional cellular processes such as response to oxidative stress by regulating cytosolic thioredoxin levels.

Pathways

Cysteinyl-tRNA synthetase plays a significant role within the translation pathway aiding in the biosynthesis of proteins by providing the essential building blocks. Another critical pathway involving CARS is the response to oxidative stress where it interacts with proteins like thioredoxin supporting cellular defense mechanisms against oxidative damage. These interactions highlight its importance in maintaining cellular homeostasis and function.

Alterations in CARS function have been associated with neurodegenerative disorders and certain types of cancer. In neurodegeneration abnormalities in cysteinyl-tRNA synthetase can lead to defective protein synthesis and oxidative stress imbalance. Additionally in specific cancers deregulation of protein synthesis pathways involving CARS and its interaction with proteins like thioredoxin contributes to tumor progression and resistance to chemotherapy. Understanding these connections aids in developing targeted therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).

See full target information CARS1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 23:902 PubMed40804742

2025

Cancer cell SPOCD1 promotes colorectal cancer liver metastasis by activating the CXCL12/CXCR4 signaling pathway in cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Peng,Lin Zhong,Lina Luo,Yongle Ju,Yan Lu,Lui Ng,Dominic Chi-Chung Foo,Fu Cheng,Luji Huang,Manzhao Ouyang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CARS antibody [CL2302]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (AB242361)

Western blot - Anti-CARS antibody [CL2302] (AB242361)

Western blot - Anti-CARS antibody [CL2302] (AB242361)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise