AB126714

Anti-CARS antibody [EPR7121]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

대체 명칭 보기

CARS, CARS1, Cysteinyl-tRNA synthetase, CysRS

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (AB126714)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (AB126714)

ab126714 at 1/50 dilution stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CARS antibody [EPR7121] (AB126714)

All lanes:

Western blot - Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

Lane 4:

BxPC-3 cell lysate

Lane 5:

MCF-7 cell lysate

Lane 6:

A549 cell lysate

Predicted band size: 85 kDa

false

OI-RD Scanning - Anti-CARS antibody [EPR7121] (AB126714)

OI-RD Scanning

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OI-RD Scanning - Anti-CARS antibody [EPR7121] (AB126714)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7121

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval step is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The CARS protein also known as cysteinyl-tRNA synthetase is an enzyme responsible for attaching cysteine to its corresponding tRNA thereby playing a vital role in protein synthesis. The molecular mass of CARS is approximately 87 kDa and it is widely expressed in various human tissues including the liver and brain. As a part of the aminoacyl-tRNA synthetase (aaRS) family it ensures that the genetic code is accurately translated into functional proteins.

Biological function summary

Cysteinyl-tRNA synthetase acts as an important component in the translation machinery ensuring incorporation of cysteine into growing polypeptide chains during protein synthesis. It forms part of the multi-synthetase complex (MSC) which is important for cellular translation and efficiency. Beyond its primary synthetase activity CARS also participates in additional cellular processes such as response to oxidative stress by regulating cytosolic thioredoxin levels.

Pathways

Cysteinyl-tRNA synthetase plays a significant role within the translation pathway aiding in the biosynthesis of proteins by providing the essential building blocks. Another critical pathway involving CARS is the response to oxidative stress where it interacts with proteins like thioredoxin supporting cellular defense mechanisms against oxidative damage. These interactions highlight its importance in maintaining cellular homeostasis and function.

Alterations in CARS function have been associated with neurodegenerative disorders and certain types of cancer. In neurodegeneration abnormalities in cysteinyl-tRNA synthetase can lead to defective protein synthesis and oxidative stress imbalance. Additionally in specific cancers deregulation of protein synthesis pathways involving CARS and its interaction with proteins like thioredoxin contributes to tumor progression and resistance to chemotherapy. Understanding these connections aids in developing targeted therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).

See full target information CARS1

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 11:eady6386 PubMed40991712

2025

Site-specific adaptive nanovesicles for oral insulin delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Bozhang Xia,Fengfei Xu,Junge Chen,Shaobo Shan,Jiuheng Shen,Yuxuan Zhang,Jinjin Wang,Ziran Zhou,Weilun Sun,Yaru Jia,Ya-Li Zhang,Jie Zhong,Gang Zhang,Yichen Wan,Zhongbo Hu,Wenlin An,Yubo Fan,Massimo Bottini,Jinqiang Wang,Xing-Jie Liang

Nature cancer 3:471-485 PubMed35484422

2022

MYCN mediates cysteine addiction and sensitizes neuroblastoma to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hamed Alborzinia,Andrés F Flórez,Sina Kreth,Lena M Brückner,Umut Yildiz,Moritz Gartlgruber,Dorett I Odoni,Gernot Poschet,Karolina Garbowicz,Chunxuan Shao,Corinna Klein,Jasmin Meier,Petra Zeisberger,Michal Nadler-Holly,Matthias Ziehm,Franziska Paul,Jürgen Burhenne,Emma Bell,Marjan Shaikhkarami,Roberto Würth,Sabine A Stainczyk,Elisa M Wecht,Jochen Kreth,Michael Büttner,Naveed Ishaque,Matthias Schlesner,Barbara Nicke,Carlo Stresemann,María Llamazares-Prada,Jan H Reiling,Matthias Fischer,Ido Amit,Matthias Selbach,Carl Herrmann,Stefan Wölfl,Kai-Oliver Henrich,Thomas Höfer,Andreas Trumpp,Frank Westermann

Frontiers in oncology 11:654076 PubMed34046350

2021

A Novel Ferroptosis Related Gene Signature for Prognosis Prediction in Patients With Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Nie,Dan Shan,Shun Li,Shuyuan Zhang,Xiaolin Zi,Fan Xing,Jiaqi Shi,Caiqi Liu,Tianjiao Wang,Xiaoyuan Sun,Qian Zhang,Meng Zhou,Shengnan Luo,Hongxue Meng,Yanqiao Zhang,Tongsen Zheng

Oncology reports 45: PubMed33846793

2021

CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixian Wang,Xiao Zhang,Xiaoting Tian,Yueyue Yang,Lifang Ma,Jiayi Wang,Yongchun Yu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CARS antibody [EPR7121]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [EPR7121] (AB126714)

Western blot - Anti-CARS antibody [EPR7121] (AB126714)

OI-RD Scanning - Anti-CARS antibody [EPR7121] (AB126714)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise