Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct) labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IP

Unknown

Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis tissue lysate 10 ug

Lane 2 : ab238121 IP in mouse testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab238121 in mouse testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121)

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (AB238121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] (ab238121) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc- tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with human Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with mouse Casein Kinase 1 gamma 2 (WT) expression vector containing a myc-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false