AB174847

Anti-Caspase-14 antibody [EPR12927]

Name: Anti-Caspase-14 antibody [EPR12927]
Brand: Abcam Limited
SKU: AB174847
Rating: 4 (1 reviews)

RabMAb
Recombinant
20ul selling size
각 태그의 뜻

(1 리뷰)

(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Caspase-14 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

대체 명칭 보기

Caspase-14, CASP-14, CASP14

6 이미지

Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Intracellular flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14using ab174847 at a 1/10 dilution (red), or rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (AB174847)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1 : 250 dilution and labeling tubulin (red) with ab7291 at 1 : 500 (2 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1 : 200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1 : 200.

-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using ab174847 at a 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1 : 10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (ab174847)

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-Caspase-14 antibody [EPR12927] (AB174847)

All lanes:

Western blot - Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

ECV-304 cell lysate at 10 µg

Lane 3:

HACAT cell lysate at 10 µg

Lane 4:

Human skin lysate at 10 µg

Predicted band size: 28 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12927

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed : 15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed : 16854378, PubMed : 19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed : 25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed : 24743736).

See full target information CASP14

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed37047109

2023

Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Seung Yeol Lee,Yoon Soo Cho,Dong Hyun Kim,Cheong Hoon Seo

Bioengineering (Basel, Switzerland) 9: PubMed36290526

2022

Keratin Promotes Differentiation of Keratinocytes Seeded on Collagen/Keratin Hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Kameel Zuniga,Neda Ghousifam,John Sansalone,Kris Senecal,Mark Van Dyke,Marissa Nichole Rylander

Scientific reports 11:17296 PubMed34453089

2021

Effect of extracorporeal shock wave therapy on keratinocytes derived from human hypertrophic scars.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Yoon Soo Cho,Ji Heon Park,Yu Mi Ro,June-Bum Kim,Cheong Hoon Seo

Proteomics. Clinical applications 13:e1900029 PubMed31282103

2019

Moonlighting Proteins and Cardiopathy in the Spatial Response of MCF-7 Breast Cancer Cells to Tamoxifen.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrab Ahmed M Alkhanjaf,Roberto Raggiaschi,Mark Crawford,Gabriella Pinto,Jasminka Godovac-Zimmermann

Cancer science 109:373-383 PubMed29178343

2017

PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78.

Applications

Unspecified application

Species

Unspecified reactive species

Chiharu Moriya,Hiroaki Taniguchi,Satoru Nagatoishi,Hisayoshi Igarashi,Kouhei Tsumoto,Kohzoh Imai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caspase-14 antibody [EPR12927]

Flow Cytometry (Intracellular) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] (AB174847)

Western blot - Anti-Caspase-14 antibody [EPR12927] (AB174847)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise