AB252413

Anti-CASPR3 antibody [EPR22727-102]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
각 태그의 뜻

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Rabbit Recombinant Monoclonal CASPR3 antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse samples.

대체 명칭 보기

CASPR3, KIAA1714, CNTNAP3, Contactin-associated protein-like 3, Cell recognition molecule Caspr3

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Caspr3/CNTNAP3 with ab252413 at 1/2000 (0.274 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse cerebrum (PMID : 26807827). The section was incubated with ab252413 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Caspr3/CNTNAP3 with ab252413 at 1/50 (10.94 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Lab

Western blot - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

The lysates were kindly provided by Prof. Zilong Qiu, Institute of Neuroscience (ION), CAS.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413) at 1/1000 dilution

Lane 1:

Wild-type mouse adult prefrontal cortex tissue lysate at 20 µg

Lane 2:

CNTNAP3 knock out mouse adult prefrontal cortex tissue lysate at 20 µg

Lane 3:

Wild-type mouse adult thalamus tissue lysate at 20 µg

Lane 4:

CNTNAP3 knock out mouse adult thalamus tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 141 kDa

Observed band size: 150 kDa

false

Lab

Western blot - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 92 seconds

All lanes:

Western blot - Anti-CASPR3 antibody [EPR22727-102] (ab252413) at 1/1000 dilution

All lanes:

Mouse P5 cerebral cortex tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 141 kDa

Observed band size: 150 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22727-102

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CASPR3 also known as contactin-associated protein-like 3 is a member of the neurexin superfamily with an approximate mass of 142 kDa. This protein typically localizes at the plasma membrane within neural tissues. CASPR3 plays a role in cell adhesion which is important for synaptic function and neural development. Expression of CASPR3 is most regularly observed in the central nervous system where it contributes to the organization of the neuronal network.

Biological function summary

CASPR3 assists in axonal guidance by interacting with other proteins to form complexes necessary for neural communication. It is part of the axon-associated contactin–Caspr complex which is essential for the formation of paranodal junctions in neurons. These junctions ensure the proper functioning of myelinated axons through the coordination of neuronal signaling affecting processes like learning and memory.

Pathways

CASPR3 interacts with molecular pathways that are integral to the maintenance of neuronal architecture and signal transmission. It is involved in the neuregulin signaling pathway which affects neural glial communication. CASPR3 works alongside proteins such as CNTN1 (Contactin 1) that further aid in neural connectivity and axonal integrity. These interactions ensure the rapid transmission of nerve impulses which is important for efficient neural processing.

CASPR3 has associations with neurological conditions such as autism spectrum disorders and schizophrenia. Alterations or malfunctions in CASPR3 can disrupt synaptic adhesion and neural processing leading to symptomatic manifestations of these conditions. CASPR3 links with other proteins related to these diseases such as CNTN1 suggesting a broader impact on the neural network that underlies cognitive and behavioral health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information CNTNAP3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CASPR3 antibody [EPR22727-102]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Immunohistochemistry (Frozen sections) - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Western blot - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

Western blot - Anti-CASPR3 antibody [EPR22727-102] (AB252413)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise