Anti-Cathepsin E antibody

• WB

CiteAb

Western blot - Anti-Cathepsin E antibody (AB36996)

Cathepsin E western blot using anti-Cathepsin E antibody ab36996. Publication image and figure legend from Ye, X., Zhu, M., et al., 2020, J Neuroinflammation, PubMed 31926553.

ab36996 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab36996 please see the product overview.

LPS suppresses the formation of autophagosomes without influencing the function of lysosomes. a N9 microglial cells were exposed to 1 μg/mL LPS for 6 h, 12 h, and 24 h. The expressions of SQSTM1, an autophagy substrate, and LAMP2, a lysosome membrane protein, were detected by western blot and quantified (b, c). d Levels of Cathepsin E (CTSE) were determined by western blot in microglial cells treated with LPS for 6 h, 12 h, and 24 h, and quantified (e). f N9 microglial cells were treated with 1 μg/mL LPS for 6 h, 12 h, and 24 h, or with 100 nM bafilomycin A1 for 6 h. The fluorescence intensity of LysoSensor was recorded with a 443-nm excitation filter and a 505-nm emission filter. g N9 microglial cells were treated with LPS in the presence or absence of 10 μμ chloroquine (CQ) for 24 h. The expression of autophagy-related proteins was measured by western blot and quantified (h, i). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs control; #p < 0.05, ##p < 0.01 vs CQ

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