Anti-Cathepsin K antibody [EPR19992]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR19992] (AB207086)

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling Cathepsin K with ab207086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human ovary cancer is observed [PMID : 27076854].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR19992] (AB207086)

Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue labeling Cathepsin K with ab207086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on osteoclasts of human osteosarcoma is observed [PMID : 17559135].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Cathepsin K antibody [EPR19992] (AB207086)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cathepsin K antibody [EPR19992] (ab207086) at 1/1000 dilution

All lanes:

Human bone giant cell tumor lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 37 kDa

Observed band size: 27 kDa,37 kDa

false

Exposure time: 1s

• WB

CiteAb

Western blot - Anti-Cathepsin K antibody [EPR19992] (AB207086)

Cathepsin K western blot using anti-Cathepsin K antibody [EPR19992] ab207086. Publication image and figure legend from Suzuki, M., Takahashi, N., et al., 2020, Sci Rep, PubMed 31937805.

ab207086 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab207086 please see the product overview.

Suppressive effects of HMW-HA on enhanced cathepsin K expression induced by CTS loading at 1 Hz and 20% elongation for 24 hours. Pre-treatment with HA dose-dependently suppressed cathepsin K mRNA expression with statistical significance at a concentration of 1 mg/mL. (A) Pre-treatment with HA significantly suppressed the enhanced cathepsin K mRNA and protein expression induced by CTS loading for 24 hours, as compared to the sample without HA pre-treatment. Cathepsin K protein expression was also enhanced with CTS loading, as compared to the untreated sample cultured for 24 hours, as demonstrated by Western blot analysis. (B) The suppressive effect of HA was cancelled by pre-treatment with anti-CD44 antibody for one hour, whereas no significant effect was observed with anti-ICAM-1 antibody pre-treatment (*p < 0.05) (C).

false