AB325402

Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal Caveolin-1 phospho Y14 antibody. Carrier free. Suitable for Dot, IP, WB and reacts with Synthetic peptide - Human, Mouse, Human samples.

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Supplier Data

Immunoprecipitation - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

This data was developed using ab325394, the same antibody clone in a different buffer formulation.

Caveolin-1 (phospho Y14) was immunoprecipitated from 0.35 mg EA.hy926 treated with 5mM H2O2 for 5 min whole cell lysate with ab325394 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325394 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] (<a href='/ko/products/primary-antibodies/caveolin-1-phospho-y14-antibody-epr28081-353-ab325394'>ab325394</a>) at 1/30 dilution

Lanes 1 - 2:

EA.hy926 treated with 5mM H2O2 for 5 min whole cell lysate at 5 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/caveolin-1-phospho-y14-antibody-epr28081-353-ab325394'>ab325394</a> in EA.hy926 treated with 5mM H2O2 for 5 min whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 20 kDa

false

Exposure time: 137s

Supplier Data

Immunoprecipitation - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

This data was developed using ab325394, the same antibody clone in a different buffer formulation.

Caveolin-1 (phospho Y14) was immunoprecipitated from 0.35 mg NIH/3T3 treated with 5mM H2O2 for 5 min whole cell lysate with ab325394 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325394 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Lanes 1 - 2:

NIH/3T3 treated with 5mM H2O2 for 5 min whole cell lysate at 5 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/caveolin-1-phospho-y14-antibody-epr28081-353-ab325394'>ab325394</a> in NIH/3T3 treated with 5mM H2O2 for 5 min whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 20 kDa

false

Exposure time: 24s

Supplier Data

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

This data was developed using ab325394, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of Caveolin-1 (phospho Y14) is upshifted in response to H2O2 treatment (PMID : 12531427).

The up-regulation of Caveolin-1 (phospho Y14) is induced by H2O2 treatment (PMID : 12531427).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Caveolin-1 antibody staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] (<a href='/ko/products/primary-antibodies/caveolin-1-phospho-y14-antibody-epr28081-353-ab325394'>ab325394</a>) at 1/1000 dilution

Lane 1:

Untreated EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

EA.hy926 treated with 5mM H2O2 for 5 min whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 4:

NIH/3T3 treated with 5mM H2O2 for 5 min whole cell lysate (untreated membrane) at 20 µg

Lane 5:

Untreated EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 6:

EA.hy926 treated with 5mM H2O2 for 5 min whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 7:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 8:

NIH/3T3 treated with 5mM H2O2 for 5 min whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

This data was developed using ab325394, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab325394 was shown to bind specifically to Caveolin-1 (phospho Y14). Target of interest was observed at 20 kDa in wild-type HeLa treated with 5mM H2O2 for 5 min lysates (lane 2) with no signal observed at this size in CAV1 knockout cell line (lanes 3-4) (lanes 3-4, knockout cell line ab255371, knockout cell lysate ab263806).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Caveolin-1 antibody staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] (<a href='/ko/products/primary-antibodies/caveolin-1-phospho-y14-antibody-epr28081-353-ab325394'>ab325394</a>) at 1/1000 dilution

Lane 1:

Untreated Wilde-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (untreated membrane) at 80 µg

Lane 2:

Wild-type HeLa treated with 5mM H2O2 for 5 min whole cell lysate (untreated membrane) at 80 µg

Lane 3:

Untreated CAV1 knockout HeLa whole cell lysate (untreated membrane) at 80 µg

Lane 4:

CAV1 knockout HeLa treated with 5mM H2O2 for 5 min whole cell lysate (untreated membrane) at 80 µg

Lane 5:

Untreated Wilde-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate (alkaline phosphatase treated membrane) at 80 µg

Lane 6:

Wild-type HeLa treated with 5mM H2O2 for 5 min whole cell lysate (alkaline phosphatase treated membrane) at 80 µg

Lane 7:

Untreated CAV1 knockout HeLa whole cell lysate (alkaline phosphatase treated membrane) at 80 µg

Lane 8:

CAV1 knockout HeLa treated with 5mM H2O2 for 5 min whole cell lysate (alkaline phosphatase treated membrane) at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

This data was developed using ab325394, the same antibody clone in a different buffer formulation.

Dot blot analysis of Caveolin-1 (phospho Y14) using ab325394 at 1000 (0.514 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : Caveolin-1 (phospho Y14) peptide a
Lane2 : Caveolin-1 non-phospho peptide b

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] (<a href='/ko/products/primary-antibodies/caveolin-1-phospho-y14-antibody-epr28081-353-ab325394'>ab325394</a>) at 1/1000 dilution

Lane 1:

Caveolin-1 (phospho Y14) peptide

Lane 2:

Caveolin-1 non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28081-353

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Dot, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab325402 is the carrier-free version of ab325394.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May act as a scaffolding protein within caveolar membranes (By similarity). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed : 19546242). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (By similarity). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (PubMed : 10816572). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (By similarity). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (PubMed : 34799735).

See full target information Cav1 phospho Y14

대체 명칭 보기

Cav, Caveolin-1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free

Immunoprecipitation - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

Immunoprecipitation - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR28081-353] - BSA and Azide free (AB325402)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise