AB235305

Anti-CBX2 antibody

Name: Anti-CBX2 antibody
Brand: Abcam Limited
SKU: AB235305
Rating: 3 (1 reviews)

(1 리뷰)

(8 제품이 사용된 논문 )

Rabbit Polyclonal CBX2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBX2 aa 1-250.

대체 명칭 보기

Chromobox protein homolog 2, CBX2

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

Paraffin-embedded human tonsil tissue stained for CBX2 using ab235305 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

Paraffin-embedded human breast cancer tissue stained for CBX2 using ab235305 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CBX2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q14781

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 'CBX2' protein also known as chromobox homolog 2 is an important component of the genetic regulation machinery. It weighs approximately 64 kDa and is mainly expressed in the nucleus of cells. CBX2 belongs to the Polycomb group (PcG) proteins specifically forming a part of the Polycomb repressive complex 1 (PRC1). Through its interaction with chromatin CBX2 induces transcriptional repression an important function for maintaining cellular identity and pluripotency.

Biological function summary

The effects of this protein extend to important roles in embryogenesis and cell cycle regulation. CBX2 serves as a part of PRC1 where it acts to mark chromatin for activation or repression. This complex allows CBX2 to recruit additional enzymes and proteins that modify chromatin structure ensuring tight regulation of gene expression. Cell differentiation depends heavily on the epigenetic control mediated by such proteins highlighting the importance of CBX2 in developmental biology.

Pathways

CBX2 plays integral roles in both the Wnt and the Hedgehog signaling pathways impacting cellular growth and differentiation. In these pathways CBX2 interacts with proteins like β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway influencing gene transcription. These interactions highlight CBX2's importance in switching genetic programs on or off in response to cellular signaling.

Researchers have associated CBX2 with cancer and sexual development disorders. Abnormal expression patterns of CBX2 are often found in various cancer types where it can interact with proteins like BMI1 to alter chromatin states. Additionally mutations in CBX2 have been linked to disorders of sex development (DSD) where it influences genes critical for sexual differentiation. Understanding CBX2's role could cast light on pathologies related to these diseases and provide potential therapeutic targets.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity). Involved in sexual development, acting as activator of NR5A1 expression (PubMed : 19361780).

See full target information CBX2

style

left-column

제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 15:1430891 PubMed39114365

2024

CBX2 enhances the progression and TMZ chemoresistance of glioma via EZH2-mediated epigenetic silencing of PTEN expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Bo Yang,Yingzhao Wang,Shuhan Liu,Changkai Ma,Jianmin Piao,Shiqiang Ma,Dehai Yu,Wei Wu

Experimental and therapeutic medicine 26:562 PubMed37954123

2023

miR‑149‑3p suppresses the proliferation and metastasis of glioma cells by targeting the CBX2/Wnt/β‑catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Wang,Yanqun Song,Zhongcheng Liu,Junmin Li,Guodong Wang,Hong Pan,Zhiming Zheng

BMC cancer 23:641 PubMed37430195

2023

An integrative bioinformatics investigation and experimental validation of chromobox family in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fenling Zhou,Lu Chen,Peng Lu,Yuli Cao,Cuilan Deng,Gexiu Liu

American journal of cancer research 13:1363-1376 PubMed37168348

2023

EBNA2 mediates lipid metabolism and tumorigenesis through activation of ATF4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Feng,Ping Zhang,Paul Yao,Hongyu Zhang

American journal of translational research 14:1188-1203 PubMed35273722

2022

Cannabidiol promotes apoptosis of osteosarcoma cells and by activating the SP1-CBX2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangxing Xu,Guiyuan Sun,Zhibin Peng,Jingsong Liu,Zecheng Li,Jinglong Yan

Technology in cancer research & treatment 20:15330338211045831 PubMed34709960

2021

CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Le Wang,Bingcheng Ren,Hao Zhuang,Yue Zhong,Yang Nan

Molecular medicine reports 23: PubMed33313949

2020

CBX2 depletion inhibits the proliferation, invasion and migration of gastric cancer cells by inactivating the YAP/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Zeng,Bangxue Li,Lei Yang,Quanlin Guan

Journal of Cancer 11:5198-5209 PubMed32742466

2020

The Prognostic Value of the Chromobox Family in Human Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yichi Xu,Shuya Pan,Yizuo Song,Chunyu Pan,Cheng Chen,Xueqiong Zhu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CBX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (8)

Product promise