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AB327602

Anti-CCKBR antibody [HL4404] - BSA and Azide free

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Rabbit Recombinant Monoclonal CCK2-R antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell lysate samples.

대체 명칭 보기

CCKRB, CCKBR, Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R

3 이미지
Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (AB327602)
  • WB

Supplier Data

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (AB327602)

Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327602 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (ab327602) at 1/500 dilution

Lane 1:

Unboiled 22Rv1 at 30 µg

Lane 2:

Unboiled 293T at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (AB327602)
  • WB

Supplier Data

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (AB327602)

Unboiled NT2D1 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327602 diluted at 1 : 1000. The ab327602 was used to detect the primary antibody. (WCE : whole cell extract; ME : membrane extract)

All lanes:

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (ab327602) at 1/1000 dilution

Lane 1:

Unboiled NT2D1 WCE at 30 µg

Lane 2:

Unboiled NT2D1 ME at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (AB327602)
  • WB

Supplier Data

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (AB327602)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327602 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CCKBR antibody [HL4404] - BSA and Azide free (ab327602) at 1/5000 dilution

Lane 1:

Boiled 293T cells at 30 µg

Lane 2:

Boiled HA-tagged CCKBR transfected 293T cells at 30 µg

Lane 3:

Unboiled 293T cells at 30 µg

Lane 4:

Unboiled HA-tagged CCKBR transfected 293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL4404

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Receptor for the peptide hormones gastrin and cholecystokinin (CCK). Expressed throughout the central nervous system, where it modulates processes such as anxiety, analgesia, arousal and neuroleptic activity. Couples to both GNAI1 and GNAQ signaling pathways, but not to GNAS (PubMed : 34556863). Upon gastrin activation, reduces glucose absorption in intestinal epithelial cells by downregulating SGLT1 and GLUT2 expression through suppression of the PI3K/Akt/eIF4B pathway (By similarity). In the kidney, decreases SGLT2 expression under high-glucose conditions via ERK/NF-kappa-B signaling (By similarity).. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
See full target information CCKBR
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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