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AB89396

Anti-CCL21 antibody [MM0146-2H26]

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Mouse Monoclonal CCL21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL21.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL21 antibody [MM0146-2H26] (AB89396)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL21 antibody [MM0146-2H26] (AB89396)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4μm) labelling CCL21 with ab89396. Tissue was treated with 15μg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Western blot - Anti-CCL21 antibody [MM0146-2H26] (AB89396)
  • WB

Supplier Data

Western blot - Anti-CCL21 antibody [MM0146-2H26] (AB89396)

All lanes:

Western blot - Anti-CCL21 antibody [MM0146-2H26] (ab89396) at 1/500 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 15 kDa

Observed band size: 12 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0146-2H26

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCL21.

O00585

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대체품

Primary Antibodies

AB9851

Anti-CCL21 antibody

Sandwich ELISA - Anti-CCL21 antibody (AB9851)

  • 4
  • 4
Primary Antibodies

AB129014

Anti-CCL21 antibody [EPR6218]

RabMAb|Recombinant

Western blot - Anti-CCL21 antibody [EPR6218] (AB129014)

  • 0
  • 0
Primary Antibodies

AB197760

Anti-CCL21 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL21 antibody (AB197760)

  • 0
  • 0

보완 제품

Assay Kits

AB193759

Human Exodus 2 ELISA Kit (CCL21)

SimpleStep

Sandwich ELISA - Human Exodus 2 ELISA Kit (CCL21) (AB193759)

  • 0
  • 0
Primary Antibodies

AB221704

Anti-MIP-3 beta/CCL19 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody (AB221704)

  • 0
  • 0
Primary Antibodies

AB281587

Anti-LYVE1 antibody [RM1008]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-LYVE1 antibody [RM1008] (AB281587)

  • 2
  • 1
Primary Antibodies

AB200699

Anti-Human IgG antibody [IG266]

BOND RX™ Validated|Lab Essentials|Recombinant

Flow Cytometry - Anti-Human IgG antibody [IG266] (AB200699)

  • 5
  • 2
Primary Antibodies

AB109489

Anti-Human IgG antibody [EPR4421]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

  • 4
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Reconstituted antibody should be aliquoted and can be stored frozen at < -20 for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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특성 및 보관 정보

제형
Lyophilized
Reconstitution
reconstitute with PBS at 200µL
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 μm filter.
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-80°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CCL21 also known as Exodus 2 or 2H26 is a chemokine with significant roles in the immune system. It is a small protein with a molecular mass of approximately 12 kDa. CCL21 is expressed in high concentrations in secondary lymphoid organs such as the spleen lymph nodes and Peyer's patches. It serves as a chemoattractant particularly for lymphocytes and dendritic cells facilitating their migration towards lymphoid organs.
Biological function summary

CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.

Pathways

CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.

CCL21 is associated with several immune-related conditions including rheumatoid arthritis and chronic lymphocytic leukemia. In rheumatoid arthritis CCL21 contributes to the infiltration and retention of immune cells in the synovial tissue while in chronic lymphocytic leukemia it aids in the homing of malignant cells to lymphoid organs. CCL21's interaction with CCR7 in these diseases highlights its importance not only in immune surveillance but also in the pathology of these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information CCL21

대체 명칭 보기

SCYA21, UNQ784/PRO1600, CCL21, C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 10:e0139603 PubMed26441059

2015

Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Wolpert,Caroline Happold,Guido Reifenberger,Ana-Maria Florea,René Deenen,Patrick Roth,Marian Christoph Neidert,Katrin Lamszus,Manfred Westphal,Michael Weller,Günter Eisele
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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