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AB194354

Anti-CCL27 antibody [EPR8583(2)]

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(1 출판물)

Rabbit Recombinant Monoclonal CCL27 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

ILC, SCYA27, CCL27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (AB194354)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (AB194354)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

Western blot - Anti-CCL27 antibody [EPR8583(2)] (AB194354)
  • WB

Supplier Data

Western blot - Anti-CCL27 antibody [EPR8583(2)] (AB194354)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution

All lanes:

Human CCL27 Recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8583(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB89543

Anti-CCL27 antibody [MM0152-7H44]

Western blot - Anti-CCL27 antibody [MM0152-7H44] (AB89543)

  • 0
  • 0
Primary Antibodies

AB181323

Anti-CCL27 antibody [EPR17284-217]

RabMAb|Recombinant

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (AB181323)

  • 0
  • 0
Primary Antibodies

AB186421

Anti-CCL27 antibody [EPR8582(2)]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

  • 0
  • 0
Primary Antibodies

AB228323

Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (AB228323)

  • 0
  • 0

보완 제품

Primary Antibodies

AB270344

Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-57] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-57] - BSA and Azide free (Capture) (AB270344)

  • 0
  • 0
Primary Antibodies

AB242823

Anti-CXCL13 antibody [EPR19259-147] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CXCL13 antibody [EPR19259-147] - BSA and Azide free (Detector) (AB242823)

  • 0
  • 0
Primary Antibodies

AB242720

Anti-Eotaxin 2 antibody [EPR17018-243] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Eotaxin 2 antibody [EPR17018-243] - BSA and Azide free (Detector) (AB242720)

  • 0
  • 0
Primary Antibodies

AB242608

Anti-CCL23 antibody [EPR19763-47] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CCL23 antibody [EPR19763-47] - BSA and Azide free (Capture) (AB242608)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.
Biological function summary

CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.

Pathways

The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.

CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
See full target information CCL27
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS genetics 18:e1010510 PubMed36477146

2022

FgSnt1 of the Set3 HDAC complex plays a key role in mediating the regulation of histone acetylation by the cAMP-PKA pathway in Fusarium graminearum.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Gong,Daiying Xu,Daiyuan Sun,Jiangang Kang,Wei Wang,Jin-Rong Xu,Xue Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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