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AB123930

Anti-CCM2 antibody [OTI2E4]

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Mouse Monoclonal CCM2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCM2.

대체 명칭 보기

C7orf22, PP10187, CCM2, Cerebral cavernous malformations 2 protein, Malcavernin

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCM2 antibody [OTI2E4] (AB123930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCM2 antibody [OTI2E4] (AB123930)

Paraffin-embedded human kidney tissue stained for CCM2 with ab123930 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody [OTI2E4] (AB123930)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody [OTI2E4] (AB123930)

pCMV6-ENTRY CCM2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for CCM2 using ab123930 at a 1/100 dilution in ICC/IF.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2E4

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCM2.

Q9BSQ5

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대체품

Primary Antibodies

AB129386

Anti-CCM2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody (AB129386)

  • 0
  • 0

보완 제품

Primary Antibodies

AB196025

Anti-KRIT1 antibody [EPR16560]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (AB196025)

  • 5
  • 3
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Assay Kits

AB66110

TUNEL Assay Kit - BrdU-Red

Flow Cytometry - TUNEL Assay Kit - BrdU-Red (AB66110)

  • 4
  • 7
Primary Antibodies

AB176569

Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Clone OTI2E4 (formerly 2E4).
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from cell culture supernatant by affinity chromatography.
보관 버퍼
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CCM2 also known as Malcavernin functions in the stability of cell-cell junctions in blood vessels. It has an approximate molecular mass of 50 kDa. The protein is largely expressed in vascular endothelial cells which are cells lining the inside of the blood vessels. CCM2 forms part of a protein scaffold that guides the cellular responses to mechanical stress maintaining vascular integrity.
Biological function summary

It interacts with several proteins to form a well-coordinated complex including KRIT1 and CCM3 contributing to the maintenance of vascular structure. The CCM2 protein is essential for regulating the cellular pathways that respond to oxidative stress. It facilitates actin cytoskeleton organization and modulates endothelial barrier function providing a structural basis for cellular processes involving vascular homeostasis.

Pathways

The protein is an important component in the RhoA signaling and MAPK pathways which influence cellular shape and proliferation. Within these pathways CCM2 influences various proteins including ROCK and MEK to execute its function. It ensures proper signal transduction that is necessary for maintaining cellular responses to environmental changes which can be important for normal vascular function.

CCM2 mutations associate closely with cerebral cavernous malformations (CCM) which trigger abnormal blood vessel formation in the brain that leads to seizures or stroke. During this condition the protein's interaction with KRIT1 exhibits significant influence since defects in the gene encoding CCM2 or KRIT1 can lead to disease phenotypes. Researchers study these interactions to better understand and develop therapeutics for vascular malformation-related diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (By similarity). May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock (By similarity).
See full target information CCM2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 287:29285-9 PubMed22782892

2012

STK25 protein mediates TrkA and CCM2 protein-dependent death in pediatric tumor cells of neural origin.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Costa,Michelle J Kean,Volker Ast,James D R Knight,Alice Mett,Zehava Levy,Derek F Ceccarelli,Beatriz Gonzalez Badillo,Roland Eils,Rainer König,Anne-Claude Gingras,Mike Fainzilber
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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