AB208778

Anti-CD10 antibody [EPR5904-110]

20ul selling size
RabMAb
Recombinant
KO Validated
각 태그의 뜻

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(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal CD10 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 6 publications.

대체 명칭 보기

CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (AB208778)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (AB208778)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (AB208778)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (AB208778)

CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.

Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : LNCaP whole cell lysate, 10μg (Input).

Lane 2 : ab208778 IP in LNCaP whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)

Predicted band size: 85 kDa

false

Lab

Western blot - Anti-CD10 antibody [EPR5904-110] (AB208778)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : CD10 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : RAJI whole cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab208778 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab208778 was shown to recognize CD10 when CD10 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD10 knockout samples were subjected to SDS-PAGE. ab208778 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-CD10 antibody [EPR5904-110] (AB208778)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/10000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 85 kDa

Observed band size: 90-110 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-CD10 antibody [EPR5904-110] (AB208778)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/1000 dilution

Lane 1:

LNCaP (Human prostate cancer cell line) whole cell lysate at 10 µg

Lane 2:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 3:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 85 kDa

Observed band size: 90-110 kDa

false

Exposure time: 15s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5904-110

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.

Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed : 15283675, PubMed : 6208535, PubMed : 6349683, PubMed : 8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed : 17101991, PubMed : 6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed : 6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed : 15283675, PubMed : 6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed : 16254193, PubMed : 2531377, PubMed : 2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).

See full target information MME

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

Biomedicines 8: PubMed33233463

2020

Three-Dimensional Biofabrication Models of Endometriosis and the Endometriotic Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian R H Wendel,Xiyin Wang,Lester J Smith,Shannon M Hawkins

Proteomics. Clinical applications 14:e1900091 PubMed31721454

2019

Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Suzuki,Atsushi Muroi,Masanori Nojima,Ayumi Numata,Hirotaka Takasaki,Rika Sakai,Tomoyuki Yokose,Yohei Miyagi,Naohiko Koshikawa

Human reproduction (Oxford, England) 34:1899-1905 PubMed31621846

2019

Different mutation profiles between epithelium and stroma in endometriosis and normal endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuaki Suda,Hirofumi Nakaoka,Kosuke Yoshihara,Tatsuya Ishiguro,Sosuke Adachi,Hiroaki Kase,Teiichi Motoyama,Ituro Inoue,Takayuki Enomoto

Brain research 1704:1-10 PubMed30253123

2018

Dendrobium polysaccharides attenuate cognitive impairment in senescence-accelerated mouse prone 8 mice via modulation of microglial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Zhuo Feng,Lin Cao,Dan Luo,Ling-Sha Ju,Jian-Jun Yang,Xin-Yi Xu,Yue-Ping Yu

Experimental and molecular pathology 104:190-198 PubMed29653092

2018

CD10 inhibits cell motility but expression is associated with advanced stage disease in colorectal cancer.

Applications

Species

Human

Teresa P Raposo,Mireia Sueca Comes,Adeyemi Idowu,Bora Agit,James Hassall,Wakkas Fadhil,Robert Nica,Rupert Ecker,Takashi Yao,Mohammad Ilyas

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD10 antibody [EPR5904-110]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (AB208778)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (AB208778)

Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (AB208778)

Western blot - Anti-CD10 antibody [EPR5904-110] (AB208778)

Western blot - Anti-CD10 antibody [EPR5904-110] (AB208778)

Western blot - Anti-CD10 antibody [EPR5904-110] (AB208778)

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise