Anti-CD101 antibody [EPR28974-15]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [EPR28974-15] (AB325180)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD101 with ab325180 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD11c conjugated to Alexa Fluor®488.
Gated on viable cells.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD101 antibody [EPR28974-15] (AB325180)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling CD101 with ab325180 at 1/50 (9.94 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in a subset of human PBMC (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/ab7291 1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab325180 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [EPR28974-15] (AB325180)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD101 with ab325180 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD8a conjugated to Pacific Blue.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [EPR28974-15] (AB325180)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD101 with ab325180 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD56 conjugated to PE.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [EPR28974-15] (AB325180)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD101 with ab325180 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with CD4 conjugated to Brilliant Violet 421.
Gated on viable cells.

• Dot

Supplier Data

Dot Blot - Anti-CD101 antibody [EPR28974-15] (AB325180)

Dot blot analysis of CD101 using ab325180 at 1000 (0.497 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : Human CD101 peptide
Lane2 : Human IGSF3 peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human IGSF3.

All lanes:

Dot Blot - Anti-CD101 antibody [EPR28974-15] (ab325180) at 1/1000 dilution

Lane 1:

Human CD101 peptide

Lane 2:

Human IGSF3 peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s