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AB235896

Anti-CD109 antibody

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Rabbit Polyclonal CD109 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD109 aa 950 to C-terminus.

대체 명칭 보기

CD109, CPAMD7, CD109 antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, Platelet-specific Gov antigen, p180, r150

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody (AB235896)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody (AB235896)

Paraffin-embedded human placenta tissue stained for CD109 using ab235896 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-CD109 antibody (AB235896)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD109 antibody (AB235896)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CD109 (green) using ab235896 at 1/66 dilution in ICC/IF, followed by an Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CD109 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6YHK3

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대체품

Primary Antibodies

AB47169

Anti-CD109 antibody [B-E47] - BSA and Azide free

Flow Cytometry - Anti-CD109 antibody [B-E47] - BSA and Azide free (AB47169)

  • 0
  • 0

보완 제품

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (AB267788)

  • 0
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Primary Antibodies

AB134131

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  • 1
Primary Antibodies

AB201811

Anti-PD1 antibody [NAT105] - BSA and Azide free

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Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811)

  • 0
  • 0
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD109 also known as platelet activation antigen 3 is a glycoprotein with a molecular weight around 170 kDa. You can find it mostly expressed on the surface of platelets activated T-cells epithelial cells and some tumor cells. This protein belongs to the α2-macroglobulin/C3 C4 C5 family and plays a part in regulating several cellular activities. CD109 acts mechanically as a glycosylphosphatidylinositol (GPI)-anchored protein which suggests its involvement in signal transduction processes.
Biological function summary

The protein CD109 modulates several cellular functions including cell proliferation differentiation and immune response regulation. The protein works as a negative regulator of TGF-β signaling by forming a complex with the TGF-β receptor. This activity influences processes like wound healing and immune regulation where TGF-β plays a vital role. CD109 expression also impacts cellular interactions and behavior in the tumor microenvironment adding additional layers of regulation to its function.

Pathways

CD109 participates in the TGF-β signaling pathway and has effects on processes like fibrosis and epithelial-mesenchymal transition (EMT). This involvement connects CD109 functionally to components such as Smad proteins which mediate the signal for TGF-β pathway downstream effects. CD109's modulation within this pathway suggests a regulatory influence that may alter cellular responses under different physiological conditions.

Research associates CD109 with various cancers where it appears to influence tumor progression and metastasis. The protein shows altered expression in glioblastoma and certain leukemias. Its involvement in these cancers links CD109 to other cancer-related proteins such as epidermal growth factor receptor (EGFR) which often shows interplay in signaling pathways involved in cell proliferation and survival. Understanding CD109's function and impact can offer insights into new therapeutic targets in oncology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Modulates negatively TGFB1 signaling in keratinocytes.
See full target information CD109
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 14:107 PubMed36774351

2023

CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Shen,Yuling Chen,Yuntao Lin,Yicun Li,Pengfei Liu,Biru Zhang,Yufan Wang,King-Chi Chan,Nai-Ki Mak,Michael Kahn,Robert Z Qi,Hongyu Yang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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