Anti-CD11b antibody [M1/70]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)

Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)

Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling YKL-40/CHI3L1 with ab326957 at 1/500 (2.07 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subsets of cd11b+ mouse PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab326957 at 1/500 dilution, followed by ab150120 at 1/1000 dilution.-ve control 2 : ab8878 at 1/200 dilution, followed by ab150081 at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling YKL-40/CHI3L1 with ab326958 at 1/500 (2.01 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subsets of cd11b+ mouse PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab326958 at 1/500 dilution, followed by ab150120 at 1/1000 dilution.-ve control 2 : ab8878 at 1/1000 dilution, followed by ab150081 at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow derived dendritic cells induced by 1ug/ml LPS for 24 hours and 200ng/ml BFA for 4 hours Untreated mouse bone marrow derived dendritic cells cells labelling IL-12 p40 with ab326571 at 1/1000 (0.509 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic staining in subsets of mouse bone marrow derived dendritic cells (shown in green) treated with 1ug/ml LPS for 24 hours then add 200 ng/ml BFA for 4 hours, showing weak staining in untreated mouse bone marrow derived dendritic cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling PGRPS with ab325131 at 1/1000 (0.522 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in mouse CD11B+ bone marrow cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse bone marrow cells labelling Lactoferrin with ab325119 at 1/50000 (0.01 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subset of CD11b+mouse bone marrows (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain CD11b at 1/200 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. (Magenta).

-ve control 1 : ab325119 at 1/50000 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab8878 at 1/200 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)

Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using ab8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : WEHI-231.(PMID : 2457584) Gated on viable cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte cells labelling LY6C with ab305229 at a 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor^® 488) (ab150081) secondary antibody at a 1/1000 dilution. Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. Nuclear DNA was labeled with DAPI (shown in blue).

Counterstained with Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/1000 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 594) (ab150160) at a 1/1000 dilution (shown in magenta).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The negative controls are as follows :

-ve control 1 : LY6C (ab305229) at a 1/100 dilution, followed by Goat anti-Rat IgG H&L (Alexa Fluor^® 594) (ab150160) secondary at a 1/1000 dilution.

-ve control 2 : Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor^® 488) (ab150081) at a 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Confocal image showing membranous staining in RAW 264.7 cells. RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Negative control : WEHI-231 (PMID : 2457584)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling 5 Lipoxygenase/5-LO with ab327006 at 1/500 (1.02 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in CD11b positive mouse PBMC (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling Proteinase 3/Myeloblastin with ab324528 at 1/200 (2.445 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in CD11b+ cells of mouse bone marrow (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab324528 at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab8878 at 1/200 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.