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AB194401

Anti-CD147 antibody [8D6]

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(4 제품이 사용된 논문 )

Mouse Monoclonal CD147 antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing BSG protein.

대체 명칭 보기

CD147, UNQ6505/PRO21383, BSG, Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, EMMPRIN, HAb18G, TCSF

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [8D6] (AB194401)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [8D6] (AB194401)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD147 using ab194401 at 2 μg/ml in immunohistochemical analysis.

Flow Cytometry - Anti-CD147 antibody [8D6] (AB194401)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD147 antibody [8D6] (AB194401)

Flow cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (ab273748) stained with ab194401 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab194401) (1x106 in 100μl at 10 μg/ml) for 30 min at 4°C.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.

Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

8D6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-Fr, IP, ICC/IF, WB, IHC-P

applications

Immunogen

Cell preparation containing BSG protein. The exact immunogen used to generate this antibody is proprietary information.

P35613

Specificity

ab194401 recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147.

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대체품

Primary Antibodies

AB205450

Alexa Fluor® 488 Anti-CD147 antibody [EPR4053]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD147 antibody [EPR4053] (AB205450)

  • 0
  • 0
Primary Antibodies

AB212057

Anti-CD147 antibody [EPR18008-67]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD147 antibody [EPR18008-67] (AB212057)

  • 0
  • 0
Primary Antibodies

AB108308

Anti-CD147 antibody [EPR4053]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR4053] (AB108308)

  • 3
  • 1
Primary Antibodies

AB222389

Anti-CD147 antibody [EPR18008-8] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (AB222389)

  • 0
  • 0
Primary Antibodies

AB666

Anti-CD147 antibody [MEM-M6/1]

BOND RX™ Validated|KO Validated

Flow Cytometry - Anti-CD147 antibody [MEM-M6/1] (AB666)

  • 4
  • 5
Primary Antibodies

AB188190

Anti-CD147 antibody [EPR18008-8]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] (AB188190)

  • 0
  • 0

보완 제품

Assay Kits

AB219631

Human EMMPRIN ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB115730

Anti-Glucose Transporter GLUT1 antibody [EPR3915]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB115730)

  • 4
  • 20
Primary Antibodies

AB267503

Alexa Fluor® 647 Anti-CD9 antibody [EPR23105-121]

RabMAb|Recombinant

Immunocytochemistry - Alexa Fluor® 647 Anti-CD9 antibody [EPR23105-121] (AB267503)

  • 0
  • 0
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Actual Band: 35kDa (non-reduced); 40kDa (reduced).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from bioreactor concentrate.
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.
Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Isoform 1. Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed : 25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed : 25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed : 21620857).. Isoform 1. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2.. Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed : 11688976, PubMed : 11943775). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed : 17127621, PubMed : 21778275, PubMed : 28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed : 25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed : 19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed : 11992541, PubMed : 12553375, PubMed : 15833850).. Isoform 2. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed : 22080952, PubMed : 26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed : 28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed : 28409866).. Isoform 2. (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA.. Isoform 2. (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA.. Isoform 2. (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection.. Isoform 2. (Microbial infection) Acts as a receptor for measles virus.. Isoform 2. (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.. Plays a role in neurite outgrowth.
See full target information BSG
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 43:114286 PubMed38796854

2024

CRACD loss induces neuroendocrine cell plasticity of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bongjun Kim,Shengzhe Zhang,Yuanjian Huang,Kyung-Pil Ko,Youn-Sang Jung,Jinho Jang,Gengyi Zou,Jie Zhang,Sohee Jun,Kee-Beom Kim,Kwon-Sik Park,Jae-Il Park

Extracellular vesicles and circulating nucleic acids 4:532-547 PubMed40357132

2023

Hepatocellular carcinoma cell-derived small extracellular vesicle-associated CD147 serves as a diagnostic marker and promotes endothelial cell angiogenesis via the PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

De-Fa Huang,Wen-Juan Zhang,Jie Chen,Zhi-Gang Jiao,Xiao-Ling Wang,Ding-Yu Rao,Wei-Song Li,Die Hu,Fang-Fang Xie,Xiao-Xing Wang,Zheng-Zhe Li,Xiao-Mei Yi,Ji-Yang Wu,Yu Jiang,Qi Wang,Tian-Yu Zhong

Cancers 15: PubMed37894290

2023

Combined Biomarker System Predicts Prognosis in Patients with Metastatic Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tatjana Khromov,Lucas Fischer,Andreas Leha,Felix Bremmer,Andreas Fischer,Henning Schliephake,Michal Amit Rahat,Phillipp Brockmeyer

Cancers 15: PubMed37509297

2023

An Assessment of the Penile Squamous Cell Carcinoma Surfaceome for Biomarker and Therapeutic Target Discovery.

Applications

Unspecified application

Species

Unspecified reactive species

George Daniel Grass,Dalia Ercan,Alyssa N Obermayer,Timothy Shaw,Paul A Stewart,Jad Chahoud,Jasreman Dhillon,Alex Lopez,Peter A S Johnstone,Silvia Regina Rogatto,Philippe E Spiess,Steven A Eschrich
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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