Anti-CD164 antibody

• ICC

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Immunocytochemistry - Anti-CD164 antibody (AB238748)

A549 (Human lung carcinoma cell line) cells stained for CD164 (Green) using ab238748 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain was DAPI (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD164 antibody (AB238748)

Paraffin-embedded human liver tissue stained for CD164 using ab238748 at 1/200 dilution in immunohistochemical analysis. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.