AB233750

Anti-CD166 antibody [3D9F1]

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(5 제품이 사용된 논문 )

Mouse Monoclonal CD166 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALCAM aa 200-400.

대체 명칭 보기

CD166, MEMD, ALCAM, CD166 antigen, Activated leukocyte cell adhesion molecule

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (AB233750)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (AB233750)

Paraffin-embedded human cervical cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (AB233750)

Paraffin-embedded human bladder cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Flow Cytometry - Anti-CD166 antibody [3D9F1] (AB233750)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD166 antibody [3D9F1] (AB233750)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-CD166 antibody [3D9F1] (AB233750)

Expected MW is 47 kDa.

All lanes:

Western blot - Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution

All lanes:

Human CD166 (aa 227-381) recombinant protein

Predicted band size: 65 kDa

false

Supplier Data

Western blot - Anti-CD166 antibody [3D9F1] (AB233750)

All lanes:

Western blot - Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 65 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3D9F1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALCAM aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q13740

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from tissue culture supernatant.

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD166 also known as ALCAM (activated leukocyte cell adhesion molecule) is a cell surface glycoprotein with a mass of approximately 105 kDa. This protein is widely expressed on various cell types including activated T-cells keratinocytes neurons and endothelial cells. In the immune system CD166 has roles in T-cell activation and migration. It contains five extracellular immunoglobulin-like domains which facilitate cell-cell interactions. Researchers often regard ALCAM-Z as an alternative form sharing structural elements with CD166 protein.

Biological function summary

CD166 mediates cell-cell adhesion and plays roles in neuronal development. It participates in the formation of synaptic junctions and affects neuronal differentiation. CD166 is also a component of cell signaling complexes influencing cell proliferation and migration. In addition it controls interactions between T-cells and dendritic cells impacting immune response. Its interactions with other adhesion molecules contribute to cellular communication and tissue integrity.

Pathways

CD166 functions within signaling pathways related to cell adhesion and migration. It is involved in the Wnt signaling pathway and its interactions regulate cell polarity and morphogenesis. CD166 interacts closely with proteins such as CD6 and Catenins impacting cellular signaling and the organization of the cytoskeleton. These pathways affect processes like embryonic development and immune cell function.

CD166 has implications in cancer and autoimmune diseases. Its dysregulation links to cancer metastasis where altered expression facilitates tumor invasion and spread. CD166 also relates to autoimmune diseases through its role in T-cell activation potentially contributing to conditions like multiple sclerosis. The interaction between CD166 and CD6 influences immune cell activation and may offer therapeutic targets for modulating immune responses in these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed : 15048703, PubMed : 15496415, PubMed : 16352806, PubMed : 23169771, PubMed : 24945728, PubMed : 7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed : 15048703, PubMed : 16352806, PubMed : 24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed : 15294938, PubMed : 16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed : 15496415, PubMed : 16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed : 29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed : 24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed : 23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed : 15496415, PubMed : 23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).. Isoform 3. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

See full target information ALCAM

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mBio 16:e0132125 PubMed40444466

2025

Lack of an atypical PDR transporter generates an immunogenic strain that drives a dysregulated and lethal immune response in murine lungs.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Winski,Peter V Stuckey,Armando M Marrufo,Georgina Agyei,Robbi L Ross,Tamanna Urmi,Sarah Chapman,Felipe H Santiago-Tirado

Clinical cancer research : an official journal of the American Association for Cancer Research 28:2020-2029 PubMed35165101

2022

Praluzatamab Ravtansine, a CD166-Targeting Antibody-Drug Conjugate, in Patients with Advanced Solid Tumors: An Open-Label Phase I/II Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Boni,Mary J Fidler,Hendrik-Tobias Arkenau,Alexander Spira,Funda Meric-Bernstam,Nataliya Uboha,Rachel E Sanborn,Randy F Sweis,Patricia LoRusso,Misako Nagasaka,Javier Garcia-Corbacho,Shadia Jalal,James J Harding,Stella K Kim,Iris H C Miedema,Danielle J Vugts,Marc C Huisman,Gerben J C Zwezerijnen,Guus A M S van Dongen,C Willemien Menke van der Houven van Oordt,Song Wang,Tam Dang,Ivan A Zein,Olga Vasiljeva,Susan K Lyman,Virginia Paton,Alison Hannah,Joyce F Liu

Environmental toxicology 37:910-924 PubMed34995009

2022

Inhibition of ALKBH5-mediated m A modification of PPARG mRNA alleviates H/R-induced oxidative stress and apoptosis in placenta trophoblast.

Applications

Unspecified application

Species

Unspecified reactive species

Yongping Guo,Wenxia Song,Yali Yang

Frontiers in cell and developmental biology 9:725071 PubMed34552931

2021

Senescent Tissue-Resident Mesenchymal Stromal Cells Are an Internal Source of Inflammation in Human Osteoarthritic Cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

Wenguang Liu,Alexander S Brodsky,Meng Feng,Yajun Liu,Jing Ding,Chathuraka T Jayasuriya,Qian Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:16552-16566 PubMed33118211

2020

Human osteoarthritis cartilage-derived stromal cells activate joint degeneration through TGF-beta lateral signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenguang Liu,Meng Feng,Chathuraka T Jayasuriya,Hang Peng,Long Zhang,Yingjie Guan,John A Froehlich,Richard M Terek,Qian Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD166 antibody [3D9F1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (AB233750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (AB233750)

Flow Cytometry - Anti-CD166 antibody [3D9F1] (AB233750)

Western blot - Anti-CD166 antibody [3D9F1] (AB233750)

Western blot - Anti-CD166 antibody [3D9F1] (AB233750)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise