AB63388

Anti-CD18 (phospho T758) antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal CD18 phospho T758 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ITGB2 pT758 aa 700 to C-terminus.

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-CD18 (phospho T758) antibody (AB63388)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD18 (phospho T758) antibody (AB63388)

Immunofluorescence analysis of HepG2 cells labelled with ab63388 at 1/500 dilution. Right hand panel was treated with immunising phosphopeptide.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ITGB2 pT758 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05107

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.

Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integrin ITGAL : ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed : 1676048, PubMed : 23775590, PubMed : 38195629). Integrin ITGAL : ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM : ITGB2 and ITGAX : ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX : ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM : ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM : ITGB2 is also a receptor for factor X. Integrin ITGAD : ITGB2 is a receptor for ICAM3 and VCAM1 (PubMed : 10438935, PubMed : 8777714, PubMed : 9841932). Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL : ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrins ITGAX : ITGB2 functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175). Integrins ITGAX : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL : ITGB2 functions as a receptor of ICAM1 by acting as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Integrin ITGAM : ITGB2/MAC-1 complex functions as a signaling receptor for the ligand receptor ICAM1, ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).

See full target information ITGB2 pT758

대체 명칭 보기

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Theranostics 15:4247-4269 PubMed40225588

2025

LRG1 inhibition promotes acute pancreatitis recovery by inducing cholecystokinin Type 1 receptor expression via Akt.

Applications

Unspecified application

Species

Unspecified reactive species

Seok Ting Lim,Xinmei Zhao,Shuqing Liu,Wenjuan Zhang,Yuanyang Tan,Nidula Mullappilly,Sandip M Swain,Mei Ling Leong,Ravisankar Rajarethinam,Kah Fei Wan,Christiane Ruedl,Rodger A Liddle,Liang Li,Xiaomeng Wang

Nature immunology 23:518-531 PubMed35354953

2022

Neutrophils direct preexisting matrix to initiate repair in damaged tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Fischer,Juliane Wannemacher,Simon Christ,Tim Koopmans,Safwen Kadri,Jiakuan Zhao,Mahesh Gouda,Haifeng Ye,Martin Mück-Häusl,Peter W Krenn,Hans-Günther Machens,Reinhard Fässler,Philipp-Alexander Neumann,Stefanie M Hauck,Yuval Rinkevich

Cancer immunology research 10:245-258 PubMed34819308

2021

C3aR Signaling Inhibits NK-cell Infiltration into the Tumor Microenvironment in Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Saravanan Nandagopal,Caiyun G Li,Yu Xu,Quaovi H Sodji,Edward E Graves,Amato J Giaccia

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD18 (phospho T758) antibody

Immunocytochemistry/ Immunofluorescence - Anti-CD18 (phospho T758) antibody (AB63388)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise