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AB238463

Anti-CD1a antibody [NA1/34]

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Mouse Monoclonal CD1A antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CD1a, T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

4 이미지
Immunohistochemistry (Frozen sections) - Anti-CD1a antibody [NA1/34] (AB238463)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD1a antibody [NA1/34] (AB238463)

IHC image of CD1a staining in a section of frozen normal human skin*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238463 (shown in green) at 1 μg/ml. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor ® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount ®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] (AB238463)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] (AB238463)

ab238463 staining CD1a in MOLT-4 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238463 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] (AB238463)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] (AB238463)

ab238463 showing negative staining for CD1a in U2OS cells (NEGATIVE CELL LINE). The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238463 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] (AB238463)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD1a antibody [NA1/34] (AB238463)

ab238463 staining CD1a in Jurkat cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238463 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

NA1/34

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB237936

Anti-CD1a antibody [rC1A/711] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [rC1A/711] - BSA and Azide free (AB237936)

  • 0
  • 0
Primary Antibodies

AB238082

Anti-CD1a antibody [rC1A/711]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [rC1A/711] (AB238082)

  • 0
  • 0
Primary Antibodies

AB217214

Anti-CD1a antibody [EP3622] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] - BSA and Azide free (AB217214)

  • 0
  • 0
Primary Antibodies

AB240411

Anti-CD1a antibody [SP157] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [SP157] - BSA and Azide free (AB240411)

  • 0
  • 0
Primary Antibodies

AB108309

Anti-CD1a antibody [EP3622]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (AB108309)

  • 0
  • 0
Primary Antibodies

AB231440

Anti-CD1a antibody [SP157]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [SP157] (AB231440)

  • 0
  • 0

보완 제품

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB270797

Anti-CD1c antibody [EPR23189-196] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

  • 4
  • 4
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
Primary Antibodies

AB256494

Anti-CD10 antibody [EPR22867-118]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (AB256494)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
Preservative: 0.02% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD1a also known as T6 antigen or CD1 is a protein with an estimated molecular mass of 37 kDa. It is expressed mainly on the surface of Langerhans cells in the skin and dendritic cells in the thymus. CD1a belongs to the CD1 family which presents lipid antigens to T cells. The protein is an important marker in immunophenotyping of these dendritic cells making it useful in flow cytometry applications.
Biological function summary

CD1a participates in the immune system by presenting lipid and glycolipid antigens to T lymphocytes including natural killer T (NKT) cells. It forms a complex with β2-microglobulin which is essential for its proper function and antigen presentation. CD1a expression helps differentiate between types of dendritic cells with CD1a-positive cells often engaging in specific responses to microbial lipid antigens.

Pathways

CD1a plays a significant role in the antigen presentation pathway particularly in lipid antigen presentation to T cells. This is important for initiating immune responses. It interacts with related proteins such as CD1b and CD1c which also present lipid antigens. CD1a has implications in innate and adaptive immunity and can influence pathways involved in microbial recognition and elimination.

CD1a is associated with certain skin conditions like Langerhans Cell Histiocytosis and psoriasis. It is involved in the pathogenesis of these diseases by affecting immune responses in the skin. In Langerhans Cell Histiocytosis CD1a-positive cells accumulate abnormally. Additionally CD1a is often studied alongside related proteins like CD207 (Langerin) to understand these conditions better.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1A
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of the National Cancer Institute 65:33-42 PubMed6993744

1980

Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

K F Bradstock,G Janossy,G Pizzolo,A V Hoffbrand,A McMichael,J R Pilch,C Milstein,P Beverley,F J Bollum
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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